Influence of Analysis of PEG Mixtures with GS-320 HQ by Composition of Eluent

The comparison of chromatograms of mixture of PEG(MW: 20000, 4000, 1000, 400) and Ethyrene glycol(EG) with Asahipak GS-320 HQ column depending on the composition ratio of water and methanol is shown Fig.1. Since GS-320 HQ has higher polarity than GF-310 HQ, with GS-320 HQ, even if it uses 100% of water for eluent, hydrophobic adsorption does not take place and PEG are separated in SEC mode.
Please refer to Influence of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent.


Sample : 0.1% each, 100micro-L
1. Poly(ethylene glycol) (Mw: 20,000)
2. Poly(ethylene glycol) (Mw: 4,000)
3. Poly(ethylene glycol) (Mw: 1,000)
4. Poly(ethylene glycol) (Mw: 400)
5. EG, Ethylene glycol

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Detector     : Shodex RI
Column temp. : 30deg-C