Effect of Sample Concentration (1) (KF-402HQ)

Figure presents the relationship between the concentration of sample and the height equivalent theoretical plate (HETP) for organic solvent SEC (GPC) semi-micro columns, GPC KF-402HQ. Lower HETP is obtained with sample concentration < 2.5% for the analysis of a lower MW compounds such as dichlorohexylphthalate.
Please refer to Effect of Sample Concentration (2) (KF-403HQ + KF-402HQ) for the analysis of compound having a wide MW distribution, such as EPON1001.

Sample : Dicyclohexyl phthalate, 10micro-L

HETP (Column length)/(Theoretical plate number)
Column       : Shodex GPC KF-402HQ (4.6mmID*250mm)         
Eluent       : THF       
Flow rate    : 0.3mL/min
Column temp. : 40deg-C
Semi-micro GPC columns = Calibration Curves for KF-400HQ Series (2) (High Molecular Weight)