Analysis of Anions in Wine (VH-anion 4D)
Please use this column with suppressor type
ion chromatography system.
WINE 
VH-anion 4D was used to quantify anions in wine. VH-anion 4D provides a rapid analysis without any influence from organic acid present in wine. Malo-lactic fermentation used during wine manufacturing makes malic acid into lactic acid. 
By using VH-anion 4D wine analysis method, the degree of malo-lactic fermentation can be also monitored. 


Sample : 50-fold diluted Wine, 20micro-L
1. 
F-
2. 
Lactic acid
3. 
Cl- 
4. 
PO43-
5.  
SO32- 
6.  
SO42- 
7. 
Tartaric acid, 
Malic acid
Columns      : Shodex WINE VH-anionG 4A (4.6mmID*10mm) + WINE VH-anion 4D (4.6mmID*150mm)
Eluent       : 5mM Na2CO3 + 12mM NaHCO3 + 3% Acetone
Flow rate    : 0.8mL/min
Detector     : Suppressed conductivity
Column temp. : 30deg-C