Analysis of Choline and Acetylcholine

Choline and acetylcholine are important components of living organism as autacoid. Choline and acetylcholine separated by RSpak DE-613 or DE-413 are converted to hydrogen peroxide (H₂O₂) by AFpak ACH-494, a column with choline oxidase and acetylcholine esterase immobilized. This hydrogen peroxide can be detected by an electrochemical detector (ECD). We recommend that DE-413 is used to reduce analytical time.



Sample :
1. CHO (Choline)
2. EHC (Ethylhomocholine)
3. ACH (Acetylcholine)

Columns      : Shodex RSpak DE-613 (6.0mmID*150mm), DE-413 (4.6mmID*150mm)
Post column  : Shodex AFpak ACH-494 (4.6mmID*10mm)
Eluent       : 0.1M Phosphoric acid + 300mg/L Sodium 1-decansulfonate
               + 65mg/L Tetramethylammonium chloride(pH8.0 adjusted by 1M NaOH)
Flow rate    : 1.0mL/min
Detector     : ECD (Electrode: Pt, 350mV SCE)
Column temp. : 37deg-C