Analysis Method of Permanent Wave Solution

RSpak NN-814 packed with polyhydroxymethacrylate gel is a column for reversed phase mode like RSpak DE-613. A small number of sulfo groups (counter ion H⁺) bonded to the packings, however, characterize NN-814. When a neutral or acidic eluent is used, acidic, neutral and basic substances in principle elute in this order. Taking this advantage, NN-814 enables complicated components such as permanent wave solution to be analyzed by simple isocratic elution with good reproducibility.
In NN-814, ion exclusion, ion exchange and reversed phase modes have effect on separation. Here, acidic substances such as TGA and DTGA elute first as they are affected mainly by the ion exclusion mode. In contrast, basic substances such as ammonia and monoethanolamine elute later as they are affected mainly by the ion exchange mode. Other components are affected mainly by the reversed phase mode.