Ketones (2) (DE-413)

RSpak DE-413 is a polymer-based column for reversed phase chromatography and has unique separation characteristics and superior chemical stability. The column can be used under the same conditions as those for ODS columns. Here, ketone standards were separated by DE-413, DE-613 and an ODS column from other manufacturer. The better separation and shorter analysis time are shown with DE-413.



Sample : 5micro-L
1. 0.2% Methyl ethyl ketone
2. 0.2% Diethyl ketone
3. 0.4% Di-n-propyl ketone
4. 0.6% Di-n-butyl ketone

Columns      : (Left, above); Shodex RSpak DE-413 (4.6mmID*150mm)
               (Left, below); Shodex RSpak DE-613 (6.0mmID*150mm)
               (Right); ODS column (4.6mmID*150mm)
Eluent       : CH3CN/H2O=50/50
Flow rate    : 1.0mL/min
Detector     : UV(262nm)
Column temp. : 40deg-C