Phenols (2)

Phenols were analyzed using RSpak DE-413 (a column for reversed phase chromatography).



Sample : 5micro-L
No.
UV(220nm) Fluorescence(Ex.225nm Em.300nm)
1. Phenol 0.5ppm 50ppm
2. 4-tert-Butylphenol 1ppm 100ppm
3. .4-Octylphenol 1ppm 100ppm
4. Nonylphenol 1ppm 100ppm
Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 1.0mL/min
Detector     : UV(220nm), Fluorescence(Ex. 225nm, Em. 300nm)
Column temp. : 40deg-C