Effect of Column Size

Two CLNpak PAE columns which have different column size are compared. PAE-2000 (20mmID x 300mm) column has 6.25 times larger cross section area than PAE-800 (8mmID x 300mm) column and large amount of sample can be loaded to PAE-800 column.

Sample :
1. 0.090mg/500micro-L Corn oil
2. 0.036mg/500micro-L Di-2-ethylhexyl phthalate
3. 0.540mg/3000micro-L Corn oil
4. 0.216mg/3000micro-L Di-2-ethylhexyl phthalate

Columns      : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone 
Flow rate    : 0.5mL/min
Column temp. : Room temp.
: PAE-2000 AC (20.0mmID*300mm)
: Acetone 
: 3.4mL/min
: Room temp.