Mono-, Di- and Trisaccharides (2)

RSpak DC-613 isa column for the analysis of saccharides by normal phase mode. Under this mode, saccharides are basically eluted in increasing order of molecular weights. An increase of organic solvent in the eluent delays the elution of saccharides.
For DC-613, it is necessary to do the analysis at 60deg-C for preventing the anomer separation.
Please compare with the case of Asahipak NH2P-50


Sample :
1. Xylose
2. Fructose
3. Glucose
4. Galactose
5. Sucrose
6. Maltose
7. Lactose
8. Melibiose
9. Maltotriose
10. Raffinose

Column       : Shodex RSpak DC-613 (6.0mmID*150mm)
Eluent       : H2O/CH3CN=20/80
Flow rate    : 1.5mL/min
Detector     : Shodex RI
Column temp. : 70deg-C