Bases, Nucleosides and Nucleotides

Asahipak GS-320 HQ (a multimode column) is suitable not only for the separation of purine bases, pyrimidine bases, nucleosides and nucleotides but also for the separation of samples of their mixtures. GS-320 HQ permits one-time separation by isocratic elution without using gradient elution.


Sample : 20micro-L
1. ATP 100micro-g/mL
2. ADP 100micro-g/mL
3. IMP 100micro-g/mL
4. AMP 50micro-g/mL
5. GMP 100micro-g/mL
6. Inosine 100micro-g/mL
7. Adenine 100micro-g/mL
8. Hypoxanthine 100micro-g/mL
9. Guanine 100micro-g/mL
10. Adenosine 100micro-g/mL

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 205mM NaH2PO4 aq./205mM H3PO4 aq.=300/7
Flow rate    : 0.6mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C