Nucleotides (10) (WA-624 pH5.5)
Nucleotides were analyzed using AXpak
WA-624 (a column
for weak anion exchange chromatography). pH of the eluent is heightened to 5.5.
Please
compare with the cases of
pH4.3 and
pH5.0
Sample :
1. 5'
CDP
2. 5'
UDP
3. 5'
ADP
4. 5'
GDP
Column : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent : 0.7M CH3COONH4 buffer(pH5.5)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 60deg-C