Saccharides and Amino Sugars (1)

Generally, ligand exchange mode columns are used with aqueous eluent, but the addition of the same cation as the counter ion enables the columns to separate samples under ion exchange mode additionally to the ligand exchange mode. Pb(NO₃)₂ aqueous solution is used as an eluent for SUGAR SP0810 because the its counter ion is Pb²⁺. This means that ligand exchange occurs between the counter ions of the packing material and the hydroxyl groups of saccharides and ion exchange occurs between the sulfo groups and the amino groups of the saccharides. Meanwhile, as Pb(NO₃)₂ contains lead, the waste liquid should be disposed of in accordance with legal procedures.


Sample :
1. Glucose
2. Galactose
3. Mannose
4. Chloride anion
5. Glucosamine
6. Galactosamine

Column       : Shodex SUGAR SP0810 (8.0mmID*300mm)
Eluent       : 20mM Pb(NO3)2(pH3.8)
Flow rate    : 0.7mL/min
Detector     : Shodex RI
Column temp. : 60deg-C