Separation of Phosphate, Phosphtite and Hypophosphite

Please use this column with suppressor type ion chromatography system.

This shows the analytical conditions to separate Phosphate(HPO₄^2-),Phosphite(HPO₃^2-) and Hypophosphite(HPO₂^2-) together with the seven common anions. Since the contamination of the eluent by CO₂ gas shortens the retention time, it is not appropriate to prepare the eluent from the concentrate solution which easily absorbs CO₂ during storage. Please be sure to prepare the eluent from the powder reagent NaHCO₃ each time and limit the period of use within 12 hours.


Sample : 20micro-L
1. F^- 2mg/L
2. HPO₂^2- 10mg/L
3. Cl^- 3mg/L
4. NO₂^- 5mg/L
5. Br^- 10mg/L
6. NO₃^- 10mg/L
7. HPO₄^2- 15mg/L
8. HPO₃^2- 10mg/L
9. SO₄^2- 10mg/L
10. Oxalic acid 10mg/L

Column       : Shodex IC SI-90 4E (4.0mmID*250mm)
Eluent       : 12mM NaHCO3 aq.
Flow rate    : 1.2mL/min
Detector     : Suppressed conductivity
Column temp. : 25deg-C