Nucleic Acids in Taste Components

Asahipak GS-320 7E is a multimode column and capable of analyzing typical nucleic acids components for taste, i.e. IMP, GMP and AMP (nucleotide), and all of their metabolites with simple isocratic elution. The main characteristic of the column is that the nine components(IMP, GMP, AMP and their respective nucleotides, purine bases and pyrimidine bases) are completely separated for approximately 30 minutes and elute in order of nucleotides, nucleosides, purine bases and pyrimidine bases.
GS-320 7E is an old type of GS-320 HQ.


Sample : 50micro-g/mL each, 20micro-L
1. IMP
2. GMP
3. AMP
4. Inosine
5. Hypoxanthine
6. Guanosine
7. Guanine
8. Adenosine
9. Adenine

Column       : Shodex Asahipak GS-320 7E (7.5mmID*250mm)
Eluent       : 10mM NaH2PO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 40deg-C