Effect of Acetonitrile Concentration on Elution Time (SZ5532)

The base material of the packing material of SUGAR SZ5532 is the gel developed for adsorption & partition mode. It is highly cross-linked and its swelling and shrinkage is very small. The counter ion is Zn²⁺. Zn²⁺ forms a complex with saccharides but, when the counter ion is Zn²⁺, saccharides are not so strongly reteined by ligand exchange mode. And, when organic solvents are added in the eluent, saccharides and sugar alcohols are reteined by adsorption & partition mode and their elution volumes become larger by inceasing organic solvent concentration.


Sample :
Rhamnose
meso-Erythritol
Glucose
Xylose
Sucrose
Sorbitol
Isomaltose
myo-Inositol
Maltotriose
Raffinose

Column       : Shodex SUGAR SZ5532 (6.0mmID*150mm)
Eluent       : CH3CN/H2O 
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 60deg-C