Saccharide and Organic Acid Analysis = Elution Volume of Saccharides and Organic Acids

Elution Volume of Saccharides and Organic Acids

Elution volume of saccharides and organic acids using SUGAR SH1011 is summeraized. When the concentration of sulfuric acid in the eluent is changed, the elution volume of organic acids changes in some degree though that of saccharides changes very little. Optimal concentration of sulfuric acid should be selected according to the sample to be analyzed.

Saccharides Sugar alcohols	(a) 5mM	(b) 20mM	(b)/(a)
Stachyose	8.59min	8.66min	100.85%
Isomaltotriose	8.85	8.90	100.59
Panose	8.94	8.98	100.48
Maltotriose	9.03	9.09	100.65
Gentiobiose	9.47	9.51	100.42
Isomaltose	9.63	9.69	100.64
Melibiose	9.77	9.82	100.51
Kojibiose	9.79	9.83	100.44
Maltose	9.80	9.86	100.60
Trehalose	9.82	9.87	100.53
Nigerose	9.87	9.87	99.99
Palatinose	9.96	9.75	97.91
Palatinit	9.96	10.04	100.77
Trehalulose	9.97	10.02	100.51
Lactose	9.99	10.05	100.55
Xylobiose	10.09	10.15	100.52
Maltitol	10.19	10.27	100.78
Lactitol	10.45	10.54	100.84
Glucose	11.32	11.37	100.47
Sorbose	11.54	11.58	100.32
myo-Inositol	11.69	11.75	100.47
D-Mannose	11.85	11.90	100.44
D(+)-Galactose	11.89	11.94	100.44
D(+)-Xylose	11.92	11.97	100.43
D(-)-Fructose	12.01	12.05	100.37
Mannitol	12.26	12.34	100.63
D(-)-Sorbitol	12.42	12.47	100.39
D(+)-Rhamnose	12.46	12.51	100.41
D(+)-Arabinose	12.74	12.79	100.40
D(-)-Ribose	13.01	13.04	100.22
D-Arabitol	13.07	13.14	100.57
Xylitol	13.25	13.33	100.61
D(+)-Fucose	13.35	13.40	100.40
meso-Erythritol	13.81	13.89	100.60
N-Acetyl-alpha-D- glucosamine	14.02	14.05	100.21
N-Acetyl-alpha-D- galactosamine	15.47	15.49	100.12

Organic Acids	(a) 5mM	(b) 20mM	(b)/(a)
Oxalic acid	8.46min	9.38min	110.90%
D-Glucuronic acid	10.24	10.39	101.46
Citric acid	10.25	10.48	102.26
alpha-Ketoglutaric acid	10.27	11.47	111.66
Maleic acid	10.28	11.77	114.46
N-Acetylneuramic acid	10.53	10.94	103.92
Tartaric acid	10.67	11.06	103.65
Pyruvic acid	11.48	12.79	111.36
Malic acid	11.74	11.93	101.63
Malonic acid	11.95	12.53	104.84
trans-Aconitic acid	12.01	12.51	104.10
Succinic acid	13.78	13.83	100.39
DL-Lactic acid	14.73	14.86	100.85
Formic acid	15.70	15.82	100.78
Acetic acid	16.90	16.93	100.18
Adipic acid	17.32	17.42	100.59
Mesaconic acid	18.28	19.02	104.07
L-Pyroglutamic acid	19.00	19.53	102.77
Propionic acid	19.45	19.54	100.40

Alcohol	(a) 5mM	(b) 20mM	(b)/(a)
Ethanol	23.43min	23.52min	100.38%

Column       : Shodex SUGAR SH1011 (8.0mmID*300mm)
Eluent       : 5mM or 20mM H₂SO₄ aq.
Flow rate    : 0.6mL/min
Detector     : Shodex RI 
Column temp. : 60deg-C