GPC Clean-up of Phthalic Acid Esters

EV · Suitable for fractionation of residual pesticides in food
· Used in the purification preocess of test solution preparation method in Shoku-Au No.1003001 (October 3ed, 2006. Japan) of thw Pharmaceutical and Food Safety Bureau, MHLW, Section 2 “Simultaneous GC/MS (LC/MS) Analysis of Agricultual Chemicals in Livestock and Marine Products”
PAE · Suitable for cleaning up water samlpes such as blood and bottom sediment
· Highly effective for Fractionation of endocrine disruptors in environmental samples

(GPC clean-up for residual pesticides in food, etc)

Product code
Product name
Particle size
(micron)
Size(mm)
ID x Length
F6090005 CLNpak EV-200 16 2.0 x 150
F6090001
F6090002		 CLNpak EV-2000
CLNpak EV-G		 16
16		 20.0 x 300
20.0 x 100
F6090003
F6090004		 CLNpak EV-2000 AC
CLNpak EV-G AC		 16
16		 20.0 x 300
20.0 x 100
Product
name		 Max. sample
load (mL)		 Max. usable
pressure
(MPa)		 Recommended
flow rate
(mL/min)
Shipping
solvent
EV-200
0.2
 2.5 0.05
Ethylacetate/Cyclohexane=3/7
EV-2000
EV-G
5
–
 2.5
–		 4.0 to 5.0
–
Ethylacetate/Cyclohexane=3/7
Ethylacetate/Cyclohexane=3/7
EV-2000 AC
EV-G AC
5
–
 2.5
–		 4.0 to 5.0
–
Acetone/Cyclohexane=3/7
Acetone/Cyclohexane=3/7

Usable eluent : Acetone/Cyclohexan=1/9 to 5/5, Ethylacetate/Cyclohexane=1/9 to 5/5

(GPC clean-up for phthalic acid esters in sediments, bioogical samples, blood, etc)

Product code
Product name
Particle size
(micron)
Size(mm)
ID x Length
F7600025
F6810022
F6714007		 CLNpak PAE-800
CLNpak PAE-2000
CLNpak PAE-G		 5
5
9		 8.0 x 300
20.0 x 300
8.0 x 50
F7600026
F6810023
F6714008		 CLNpak PAE-800 AC
CLNpak PAE-2000 AC
CLNpak PAE-G AC		 5
5
9		 8.0 x 300
20.0 x 300
8.0 x 50
Product
name		 Max. sample
load(mL)		 Max. usable
pressure
(MPa)		 Recommended
flow rate
(mL/min)
Shipping
solvent
PAE-800
PAE-2000
PAE-G
0.5
3
–
 4.0
5.0
–		 0.8
3.5
–
Acetonitrile
Acetonitrile
Acetonitrile
PAE-800 AC
PAE-2000 AC
PAE-G AC
0.5 (water:10%)
3 (water:10%)
–
 4.0
5.0
–		 0.8
3.5
–
Acetone
Acetone
Acetone

Usable eluent : Acetone, Acetonitrile

CLNpak PAE-800 was used for GPC clean-up of phthalic acid esters. Compared with the case of analysis with acetone eluent, the saparation of peak 1 and peak 3 to 14 is insufficient.

Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
3-14. Phthalic acid esters
14. Dimethyl phthalate
15. H2O
16. CO2
17. Sulfur

Column       : Shodex CLNpak PAE-800 (8.0mmID*300mm)
Eluent       : Acetonitrile
Flow rate    : 0.5mL/min
Column temp. : Room temp.

CLNpak PAE-2000 was used for GPC clean-up of phthalic acid esters. Please compare with the case of analysis using PAE-800 and analysis with acetone eluent.

Sample : 1mL, dissolved in acetonitrile
1. Corn oil
2. Butylbenzyl phthalate
3. H2O
4. Sulfur
5-16. Phthalic acid esters
5. Di-ethylhexyl phthalate, 6. Di-n-octyl phthalate, 7. Di-heptyl phthalate, 8. Di-n-hexyl phthalate,
9. Di-pentyhl phthalate, 10. Di-isobutyl phthalate, 11. Di-n-butyl phthalate, 12. Di-n-propyl phthalate,
13. Di-cyclohexyl phthalate, 14. Butylbenzyl phthalate, 15. Di-ethyl phthalate, 16. Di-methyl phthalate

Columns      : Shodex CLNpak PAE-G (8.0mmID*50mm) + PAE-2000 (20.0mmID*300mm)
Eluent       : Acetonitrile
Flow rate    : 4.0mL/min
Detector     : UV(254nm),RI
Column temp. : 40deg-C

CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters. Compared with the case of analysis with acetonitrile eluent, the saparation of peak 1 and peak 3 to 14 is better.

Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
3-14. Phthalic acid esters
14. Dimethyl phthalate
15. Sulfur
16. H2O
17. CO2

Column       : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 0.5mL/min
Column temp. : Room temp.

CLNpak PAE-2000 AC was used for GPC clean-up of phthalic acid esters. Please compare with the case of analysis using PAE-800 AC and analysis with acetonitorile eluent.

Sample : 1mL, dissolved in acetone
1. Corn oil
2. Butylbenzyl phthalate
3. Sulfur
4. H2O
5-16. Phthalic acid esters
5. Di-ethylhexyl phthalate, 6. Di-n-octyl phthalate, 7. Di-heptyl phthalate, 8. Di-n-hexyl phthalate,
9. Di-pentyhl phthalate, 10. Di-isobutyl phthalate, 11. Di-n-butyl phthalate, 12. Di-n-propyl phthalate,
13. Di-cyclohexyl phthalate, 14. Butylbenzyl phthalate, 15. Di-ethyl phthalate, 16. Di-methyl phthalate
17. beta-carotene
18. Acetonitorile

Columns      : Shodex CLNpak PAE-G AC (8.0mmID*50mm) + PAE-2000 AC (20.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 4.0mL/min
Column temp. : 40deg-C

CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters. Using marker samples having UV absoption, the position of fractionating phthalic acid esters can be determined.

Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
4. Dimethyl phthalate
5. beta-carotene (dissolved in acetone)
6. Acetonitrile

Column       : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 0.5mL/min
Column temp. : Room temp.

CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters.

Sample :
1. Corn oil
2. Sulfur
3. H2O
4. CO2
5. Bis(2-ethylhexyl) adipate
6. Phthalic acid esters
7. 4-Nonylphenol
8. DDT
9. Bisphenol A
10. Antracene

Column       : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 0.5mL/min
Detector     : UV(330nm), RI
Column temp. : Room temp.

Two CLNpak PAE columns which have different column size are compared. PAE-2000 (20mmID x 300mm) column has 6.25 times larger cross section area than PAE-800 (8mmID x 300mm) column and large amount of sample can be loaded to PAE-800 column.

Sample :
1. 0.090mg/500micro-L Corn oil
2. 0.036mg/500micro-L Di-2-ethylhexyl phthalate
3. 0.540mg/3000micro-L Corn oil
4. 0.216mg/3000micro-L Di-2-ethylhexyl phthalate

Columns      : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone 
Flow rate    : 0.5mL/min
Column temp. : Room temp.

: PAE-2000 AC (20.0mmID*300mm)
: Acetone 
: 3.4mL/min
: Room temp.