SHODEX™ Columns for BioChromatography

Columns and Applications

Shodex is a world leader in polymer based column technology. This section contains extensive information on Shodex columns and their applications. Additional information is always available on request.

General Information on Packed Columns

Analytical & Preparative columns

Reversed Phase, Hydrophilic Interaction, Normal Phase

Column Selection

Characteristics and Application of Reversed Phase, Hydrophilic Interaction and Normal Phase Chromatography Columns

Column Specification

Polymer-based Columns for Reversed Phase Chromatography (RSpak)
Polymer-based Columns Reversed Phase Chromatography (Asahipak)
Polymer-based Columns for Reversed Phase Chromatography (ODP2 HP)
Polymer-based Column for HighTtemperature Analysis (ET-RP1)
Polymer-based Column for Hydrophilic Interaction Chromatography (HILIC)
Silica-based Columns (ODS Columns)
Silica-based Columns (Others)

Explanation

Sample preparation for Normal and Reversed Phase Chromatography
Eluent Conditions for RSpak D-series
Eluent Conditions for RSpak NN-series
Eluent Conditions for Asahipak (ODP, C8P, C4P, NH2P)
Line Filter NH2P-LF
Features of RSpak DE Series
Feature of High Temperature Analysis

Chiral

Column Information

Selection of Chiral Separation Columns

Column Specifications

Chiral Separation Columns (ORpak CD)
Chiral Separation Columns (ORpak CRX-853)
Chiral Separation Columns (AFpak ABA-894)

Ion Exchange

Column Specification

Ion Exchange Chromatography Columns
Ion Exchange Chromatography Columns for UHPLC

Column Information

Titration Curve of IEC QA-825
Titration Curve of IEC DEAE-825
Titration Curve of IEC SP-825
Titration Curve of IEC CM-825
Eluent Conditions for IEC-series and AXpak
Eluent Conditions for Asahipak ES-series
Features of Non-Porous Gel

Hydrophobic Interaction

USP Column List

Sample Pre-Treatment

Column Switching

Columns for Column Switching Method

GPC Clean-up

Columns for GPC Clean-up

Other

Calibration Standards

Application Information

Proteins, Peptides and Amino Acids

Guidelines for Shodex Column Selection : Proteins, Peptides and Amino acids
GFC Analysis
Reversed Phased Analysis
Ion Exchange Analysis
Fast Ion Exchange Analysis
Hydrophobic Interaction Analysis
Multimode Analysis
Affinity Chromatography Analysis
Amino Acid Analysis

Nucleic Acids

Guidelines for Shodex Column Selection : Nucleic Acids
Features of Multimode Columns (Nucleic Acid Analysis)
Purine Bases, Pyrimidine Bases, Nucleosides and Nucleotides
Nucleotides and Isomers
Oligonucleic Acids
DNA, RNA

Saccharides and Organic Acids

Guidelines for Shodex Column Selection : Saccharides and Organic Acids
Monosaccharides and Oligosaccharides
Oligosaccharides
Acid Saccharides, Phosphorylated Saccharides
Saccharides, Sugar Alcohols, Aminosugars and Amino Acids
Saccharides and Organic Acids
Saccharides and Sweetning agents(Additives for Foods)
Others
Polysaccharides
High-sensitive, High-selective Analysis of Saccharides
Organic Acids

Drugs, Metabolites and Chiral Compounds

Guidelines for Shodex Column Selection : Drugs, Metabolites and Chiral Compounds
Drugs
Drugs in Blood and Urine
Metabolites
Chiral Compounds

Vitamins, Hormones and Lipids

Guidelines for Shodex Column Selection : Vitamins, Hormones and Lipids
Vitamins
Hormones
Lipids

Pesticide Residue and Endocrine Disruptors

Agricultural Chemicals
N-Methylcarbamates
Other Pesticides
GPC Clean-up of Pesticides Residuals in Foods
GPC Clean-up of Phthalic Acid Esters
Bisphenol A
Environmental Pollutants

Anions and Cations

Guidelines for Shodex Column Selection
Anions(Non-suppressor Method: I-524A Column)
Anions(Non-suppressor Method: NI-424 Column)
Anions(Non-suppressor Method: Other Columns)
Anions(Suppressor Method: SI-90, SI-50, SI-52 Columns)
Cations(Y-521)
Cations(Simultaneous separation of monovalent and divalent cations: YS-50, YK-421)
Transition Metal Ions and Rare Earth Metal Ions

Column Switching

Columns for Column Switching
System Diagram
Ratio of Deproteinization (PK-2A)
Recovery of Various Drugs (PK-2A)
Analysis of Caffeine in BSA
Examination of Pretreatment Conditions (1) Dependency on Flow Rate
Examination of Pretreatment Conditions (2) Dependency on Quantity of Eluent
On-line Surfactant Removal (MSpak PK&GF)
BSA Analysis in Surfactant (On-line Surfactant Removal)

Other Applications

Gelatin / Albumin
Choline and Acetylcholine / Taste Components / Liquid for Permanent Wave
Surfactants
Cyanide
Alcohols / Amino Alcohols / Ketones
Additives for Foods and Drugs / Aromatic Compounds
Mycotoxins
Others

Column News

SB-807: GFC Analysis of polymers > 15 Million Daltons

Click here to view PDF

Request information on Shodex Columns for your Laboratory

SEARCH FOR A SHODEX COLUMN

Shodex columns have three identifying numbers, a model number like KW-802.5, a catalog number starting with the letter “F” like F6989109 and a Thomson number like 005-0158.

SEARCH FOR A SHODEX COLUMN

Enter any one of these numbers (whole or partial) and the Search function will find the relevant columns. Shodex columns found on HPLC.com reflect a 6% discount.

Old	New	Part Number	Description	List Price	Web Price
C18-4D	F6651010		C18-4D, F6651010 ––	330.00	310.2
C18-4E	F6651011		C18-4E, F6651011 ––	402.00	377.88
C18M 4D	F6650040	005-0050	C18M 4D, F6650040 –– Silica, Normal/reversed phase column, 4.6 x 150	593.00	557.42
C18M 4E	F6650041	005-0051	C18M 4E, F6650041 –– Silica, Normal/reversed phase column, 4.6 x 250	742.00	697.48
C18U 2B	F6654011		C18U 2B, F6654011 ––	946.00	889.24
C18U 2D	F6654012		C18U 2D, F6654012 –– UHP RP Column, 1.9µm, 2 x 150mm	1,107.00	1040.58
C4P-50 4D	F7620008	005-0038	C4P-50 4D, F7620008 –– Asahipak, Normal/reversed phase column, 4.6 x 150	931.00	875.14
C4P-50G 4A	F6710003	005-0040	C4P-50G 4A, F6710003 –– Asahipak, Normal/reversed phase column, 4.6 x 10	441.00	414.54
CDBS-453	F7146003	005-0145	CDBS-453, F7146003 –– ORpak, Chiral column, 4.6 x 150	1,194.00	1122.36
CM-825	F6110002	005-0077	CM-825, F6110002 –– IEC, Ion exchange column, 8 x 75	1,120.00	1052.8
DC-613	F7001003	005-0016	DC-613, F7001003 –– RSpak, Normal/reversed phase column, 6 x 150	944.00	887.36
DC-G 4A	F6700170	005-0017	DC-G 4A, F6700170 ––	472.00	443.68
DE-213	F7001007	005-0275	DE-213, F7001007 –– RSpak, Normal/reversed phase column, LC/MS, rapid analysis column, 2 x 150	732.00	688.08
DE-413	F7001005	005-0009	DE-413, F7001005 –– RSpak, Normal/reversed phase column, LC/MS rapid analysis, 4.6 x 150	758.00	712.52
DE-613	F7001004	005-0008	DE-613, F7001004 –– RSpak, Normal/reversed phase column, 6 x 150	831.00	781.14
DE-G 2A	F6700151	005-0013	DE-G 2A, F6700151 ––	441.00	414.54
DE-G 4A	F6700150	005-0012	DE-G 4A, F6700150 –– –– ––	441.00	414.54
DEAE-825	F6118255	005-0073	DEAE-825, F6118255 –– IEC, Ion exchange column, 8 x 75	1,120.00	1052.8
DEAE3N-4T	F801552	005-0911	DEAE3N-4T, F801552 ––	1,120.00	1052.8
DM-614	F7001002	005-0014	DM-614, F7001002 –– RSpak, Normal/reversed phase column, 6 x 150	882.00	829.08
DM-G 4A	F6700160	005-0015	DM-G 4A, F6700160 ––	441.00	414.54
DS-413	F7001012	005-0006	DS-413, F7001012 –– RSpak, Normal/reversed phase column, 4.6 x 150	745.00	700.3
DS-613	F7001001	005-0005	DS-613, F7001001 –– RSpak, Normal/reversed phase column, 6 x 150	896.00	842.24
DS-G	F6700140	005-0007	DS-G, F6700140 –– RSpak, Normal/reversed phase column, 4.6 x 10	441.00	414.54
EP SC1011-7F	F6379300		EP SC1011-7F, F6379300 –– Contact us for pricing and availability	0.00	0
ES-502C 7C	F7640001	005-0079	ES-502C 7C, F7640001 –– Asahipak, Ion exchange column, 7.6 x 100	1,308.00	1229.52
ES-502N 7C	F7640002	005-0078	ES-502N 7C, F7640002 –– Asahipak, Ion exchange column, 7.6 x 100	1,308.00	1229.52
EV-200	F6090005	005-0285	EV-200, F6090005 –– CLNpak, GPC clean-up column, 2 x 150	1,486.00	1396.84
EV-2000	F6090001	005-0286	EV-2000, F6090001 –– CLNpak, GPC clean-up column, 20 x 300	3,172.00	2981.68
EV-2000 AC	F6090003	005-0288	EV-2000 AC, F6090003 –– CLNpak, GPC clean-up column, 20 x 300	3,172.00	2981.68
EV-G	F6090002	005-0287	EV-G, F6090002 –– CLNpak, GPC clean-up column, 20 x 100	1,271.00	1194.74
EV-G AC	F6090004	005-0289	EV-G AC, F6090004 –– CLNpak, GPC clean-up column, 20 x 100	1,271.00	1194.74
EXP™	F6709801		EXP™, F6709801 –– UHP RP Cartridges, 1.9µm, 2 x 150mm, Pk/3	1,022.00	960.68
EXP™	F6709802		EXP™, F6709802 –– Direct Connect Cartridge Holder, Pk/1	821.00	771.74
FL-1	F8500630	005-0271	FL-1, F8500630 – IC, Ion chromatography line filter, Holder + 1-filter	316.00	297.04
FL-1 filter	F8500640	005-0272	FL-1, F8500640 – IC, Ion chromatography line filter, Replacement Filter	151.00	141.94
GF-1G 7B	F6710018	005-0169	GF-1G 7B, F6710018 –– Asahipak, Multisolvent column, 7.6 x 50	504.00	473.76
GF-210 HQ	F7600000		GF-210 HQ, F7600000 ––	2,078.00	1953.32
GF-310 4D	F7600110	005-0279	GF-310 4D, F7600110 –– MSpak, LC/MS, rapid analysis column, 4.6 x 150	1,414.00	1329.16
GF-310 HQ	F7600001	005-0165	GF-310 HQ, F7600001 –– Asahipak, Multisolvent column, 7.5 x 300mm	2,078.00	1953.32
GF-4A	F8700015	005-0307	GF-4A, F8700015 –– MSpak, , 4.6 x 10	670.00	629.8
GF-510 HQ	F7600002	005-0166	GF-510 HQ, F7600002 –– Asahipak, Multisolvent column, 7.6 x 300	2,078.00	1953.32
GF-7M HQ	F7600004	005-0168	GF-7M HQ, F7600004 –– Asahipak, Multisolvent column, 7.6 x 300	2,078.00	1953.32
GS-220 HQ	F7600005	005-0136	GS-220 HQ, F7600005 –– Asahipak, Sugar column, 7.5 x 300mm	2,078.00	1953.32
GS-2G 7B	F6710019	005-0137	GS-2G 7B, F6710019 –– Asahipak, Sugar guard column, 7.6 x 50mm	504.00	473.76
GS-320 HQ	F7600006	005-0162	GS-320 HQ, F7600006 –– Asahipak, Multimode column, 7.6 x 300	2,078.00	1953.32
HK-401	F6025010		HK-401, F6025010 ––	2,228.00	2094.32
HK-404L	F6026040		HK-404L, F6026040 ––	2,228.00	2094.32
HK-405	F6025050		HK-405, F6025050 ––	2,228.00	2094.32
HK-G (housing/filter)	F6700200		HK-G (housing/filter), F6700200 ––	316.00	297.04
HK-G FILTER (3 pc replace)	F6700100		HK-G FILTER (3 pc replace), F6700100 ––	441.00	414.54
I-524A	F6995240	005-0261	I-524A, F6995240 –– IC, Ion chromatography column, 4.6 x 100	1,202.00	1129.88
IA-G	F6700400	005-0262	IA-G, F6700400 –– IC, Ion chromatography guard column, 4.6 x 10	441.00	414.54
JJ-50 2D	F7008220	005-0023	JJ-50 2D, F7008220 –– RSpak, Normal/reversed phase column, LC/MS, rapid analysis column, 2.0 x 150mm	996.00	936.24
KC-811	F6378030	005-0139	KC-811, F6378030 –– RSpak, Organic acid column, 8 x 300	1,605.00	1508.7
KC-811 6E	F6378033		KC-811 6E, F6378033 ––	1,558.00	1464.52
KC-G 6B	F6700030	005-0140	KC-G 6B, F6700030 ––	472.00	443.68
KC-G 8B	F6700010	005-0141	KC-G 8B, F6700010 ––	498.00	468.12
KD-801	F6028210	005-0200	KD-801, F6028210 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-802	F6028220	005-0201	KD-802, F6028220 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-802.5	F6028225	005-0202	KD-802.5, F6028225 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-803	F6028230	005-0203	KD-803, F6028230 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-804	F6028240	005-0204	KD-804, F6028240 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-805	F6028250	005-0205	KD-805, F6028250 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-806	F6028260	005-0206	KD-806, F6028260 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-806M	F6028290	005-0207	KD-806M, F6028290 –– GPC, Gel permeation column, 8 x 300, In DMF	1,547.00	1454.18
KD-807	F6028270	005-0208	KD-807, F6028270 –– GPC, Gel permeation column, 8 x 300, In DMF	1,486.00	1396.84
KD-G 4A	F6700411	005-0209	KD-G 4A, F6700411 –– GPC, Gel permeation guard column, 4.6 x 10, In DMF	503.00	472.82
KF-401 HQ	F6028111	005-0220	KF-401 HQ, F6028111 –– GPC, Gel permeation column, 4.6 x 250, In THF	1,439.00	1352.66
KF-402 HQ	F6028112	005-0221	KF-402 HQ, F6028112 –– GPC, Gel permeation column, 4.6 x 250, In THF	1,439.00	1352.66
KF-402.5 HQ	F6028114	005-0222	KF-402.5 HQ, F6028114 –– GPC, Gel permeation column, 4.6 x 250, In THF	1,439.00	1352.66
KF-403 HQ	F6028116	005-0223	KF-403 HQ, F6028116 –– GPC, Gel permeation column, 4.6 x 250, In THF	1,439.00	1352.66
KF-800D	F6709350	005-0227	KF-800D, F6709350 –– GPC, Gel permeation column, 8 x 100, In THF	731.00	687.14
KF-801	F6028010	005-0170	KF-801, F6028010 –– GPC, Gel permeation column, 8 x 300, In THF	1,486.00	1396.84
KF-802	F6028020	005-0171	KF-802, F6028020 –– GPC, Gel permeation column, 8 x 300mm, In THF	1,486.00	1396.84
KF-802.5	F6028025	005-0172	KF-802.5, F6028025 –– GPC, Gel permeation column, 8 x 300mm, In THF	1,486.00	1396.84
KF-803	F6028030	005-0173	KF-803, F6028030 –– GPC, Gel permeation column, 8 x 300, In THF	1,486.00	1396.84
KF-803L	F6027030	005-0174	KF-803L, F6027030 –– GPC, Gel permeation column, 8 x 300, In THF	1,547.00	1454.18
KF-804	F6028040	005-0175	KF-804, F6028040 –– GPC, Gel permeation column, 8 x 300, In THF	1,486.00	1396.84
KF-804L	F6027040	005-0176	KF-804L, F6027040 –– GPC, Gel permeation column, 8 x 300, In THF	1,547.00	1454.18
KF-805	F6028050	005-0177	KF-805, F6028050 –– GPC, Gel permeation column, 8 x 300, In THF	1,486.00	1396.84
KF-805L	F6027050	005-0178	KF-805L, F6027050 –– GPC, Gel permeation column, 8 x 300, In THF	1,547.00	1454.18
KF-806M	F6028090	005-0180	KF-806M, F6028090 –– GPC, Gel permeation column, 8 x 300, In THF	1,547.00	1454.18
KF-807L	F6027070	005-0183	KF-807L, F6027070 –– GPC, Gel permeation column, 8 x 300, In THF	1,547.00	1454.18
KF-G 4A	F6700300	005-0184	KF-G 4A, F6700300 ––	503.00	472.82
KS-801	F6378010	005-0129	KS-801, F6378010 –– SUGAR, Sugar column, 8 x 300	1,421.00	1335.74
KS-802	F6378020	005-0130	KS-802, F6378020 –– SUGAR, Sugar column, 8 x 300	1,421.00	1335.74
KS-803	F6378025	005-0131	KS-803, F6378025 –– SUGAR, Sugar column, 8 x 300	1,421.00	1335.74
KS-804	F6378035	005-0132	KS-804, F6378035 –– SUGAR, Sugar column, 8 x 300	1,421.00	1335.74
KS-G 6B	F6700020	005-0135	KS-G 6B, F6700020 ––	472.00	443.68
KW-802.5	F6989000	005-0157	KW-802.5, F6989000 –– PROTEIN, Gel filtration column, 5µm, 150Å, 5-150K, silica, 8 x 300mm	1,409.00	1324.46
KW-803	F6989103	005-0158	KW-803, F6989103 –– PROTEIN, Gel filtration column, 5µm, 300Å, 10-700K, silica, 8 x 300mm	1,409.00	1324.46
KW-804	F6989104	005-0159	KW-804, F6989104 –– PROTEIN, Gel filtration column, 5µm, 500Å, 10-2,000K, silica, 8 x 300mm	1,409.00	1324.46
KW-G 6B	F6700131	005-0161	KW-G 6B, F6700131 –– PROTEIN, Gel filtration guard column, 6 x 50mm	516.00	485.04
KW400G-4A	F6700132	005-0457	KW400G-4A, F6700132 –– High Performance Semi-Micro GFC Guard Column, 5µm,4.6 x 10mm	443.00	416.42
KW402.5-4F	F6989201	005-0453	KW402.5-4F, F6989201 –– High Performance Semi-Micro GFC Column, 3µm, 400Å 150K, 4.6 X 300mm	1,640.00	1541.6
KW403-4F	F6989202	005-0454	KW403-4F, F6989202 –– PROTEIN, Gel filtration column, 3µm, 800Å, 600K, 10-700K, silica, 4.6 x 300mm	1,640.00	1541.6
KW404-4F	F6989203	005-0455	KW404-4F, F6989203 –– High Performance Semi-Micro GFC Column, 5µm, 1500Å, 1000K*, 4.6 x 300mm	1,640.00	1541.6
KW405-4F	F6989204	005-0456	KW405-4F, F6989204 –– High Performance Semi-Micro GFC Column, 5µm, 2000Å, 20,000K*, 4.6 x 300mm	1,640.00	1541.6
LB-802.5	Special order		LB-802.5, Special order ––	2,016.00	1895.04
LB-803	F6429201		LB-803, F6429201 ––	2,016.00	1895.04
LB-804	F6429204		LB-804, F6429204 ––	2,016.00	1895.04
LB-805	F6429203		LB-805, F6429203 ––	2,016.00	1895.04
LB-806	Special order		LB-806, Special order ––	2,016.00	1895.04
LB-806M	F6429202		LB-806M, F6429202 ––	2,072.00	1947.68
LB-G 6B	F6709434		LB-G 6B, F6709434 ––	606.00	569.64
LF-404	F6021043	005-0444	LF-404, F6021043 –– GPC, Gel Permeation column, 4.6 x 250mm	1,737.00	1632.78
LF-604	F6021042	005-0443	LF-604, F6021042 –– GPC, Gel Permeation column, 6 x 150mm	1,737.00	1632.78
LF-804	F6021041	005-0210	LF-804, F6021041 –– GPC, Gel permeation column, 8 x 300	1,737.00	1632.78
LF-G	F6709621	005-0445	LF-G, F6709621 –– GPC, Gel Permeation column, 4.6 x 10mm	503.00	472.82
LW-403 4D	F6989403		LW-403 4D, F6989403 ––	1,685.00	1583.9
LW-803	F6989303		LW-803, F6989303 ––	1,794.00	1686.36
LW-G 6B	F6700133		LW-G 6B, F6700133 ––	502.00	471.88
M-75 (0.5g x 7)	F8604075	005-0425	M-75 (0.5g x 7), F8604075 ––	2,024.00	1902.56
MN-431	F6379230	005-0138	MN-431, F6379230 –– USPpak, Sugar column, 4 x 250m