Ratio of Deproteinization (PK-2A)

The ratio of deproteinization with MSpak PK series ( Columns for Column switching ) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, PK-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis.

Ratio of Deproteinization

Sample: 200mg/mL BSA 100micro-L

Pretreatment
Eluent         : 10mM CH3COONH4(pH7.0)
Flow rate      : 0.5mL/min
Column temp.   : Room temp.
Switching time : 5min
 
Analysis 
Column       : Shodex PROTEIN KW-604S (6.0mmID*50mm)
Eluent       : 0.1% TFA in (H2O/CH3CN=50/50)
Flow rate    : 0.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.