Ion Exchange Chromatography Columns
To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.
| Column type |
Test conditions
(1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector |
|---|---|
| SP-825 | (1)0.5% Acetone (2)20micro-L (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| SP-420N | (1)0.2% Glycyl-L-tyrosine (2)1micro-L (3)20mM Acetate buffer + 0.5M Na2SO4(pH5.0) (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| CM-825 | (1)0.5% Acetone (2)20micro-L (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| ES-502C 7C | (1)0.025% Cytosine (2)15micro-L (3)100mM Sodium phosphate buffer(pH4.4) (4)1.0mL/min (5)30deg-C (6)UV(250nm) |
| P-421S | (1)1% Ethylene glycol (2)4micro-L (3)H2O (4)0.5mL/min (5)Ambient (6)RI |
| QA-825 | (1)0.5% Acetone (2)20micro-L (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| DEAE-825 | (1)0.5% Acetone (2)20micro-L (3)50mM Na2SO4 aq. (4)1.0mL/min (5)Ambient (6)UV(280nm) |
| DEAE3N-4T | (1)200mg/L NaNO3 (2)5micro-L (3)30mM phosphate buffer(pH6.0) + 0.2M NaCl (4)1.0mL/min (5)Ambient (6)UV(210nm) |
| ES-502N 7C | (1)0.025% Xanthine (2)10micro-L (3)50mM 1,3-Diaminopropane + 50mM NaCl(pH10.0) (4)1.0mL/min (5)30deg-C (6)UV(250nm) |
| WA-624 | (1)0.03% UMP (2)10micro-L (3)0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20 (4)1.0mL/min (5)Ambient (6)UV(260nm) |