Recovery of Proteins and Enzyme Activity (PH-814)
Recovery of proteins and enzyme activity were measured using HIC PH-814 ( a hydrophobic interaction column ). With the exception of myoglobin, which had a recovery of 93%, all proteins show good recovery values. Enzyme activity for all proteins remained high, except for catalase which had a value of 88% and hexokinase which had a value of 94%.
Recovery of proteins | |
---|---|
Protein | Recovery(%) |
Cytochrome c | 104 |
Myoglobin | 93 |
Ribonuclease A | 100 |
Ovalbumin | 103 |
Lysozyme | 100 |
alpha Chymotrypsin | 99 |
alpha Chymotrypsinogen A | 96 |
Recovery of enzyme activity |
|
---|---|
Enzyme | Recovery(%) |
Trypsin | 96 |
Hexokinase | 94 |
Catalase | 88 |
Lysozyme | 100 |
Lipoxidase | 100 |
Sample load : 0.5mg each
Column : Shodex HIC PH-814