Lysozyme (1)

AFpak AGA-894 ( an affinity column ) which has N-acetyl-glucosamine as the ligand was used for the adsorption and elution of lysozyme.

Sample : Lysozyme

Column       : Shodex AFpak AGA-894 (8.0mmID*50mm)
Eluent       : (A): 0.01M TRis-HCl buffer(pH7.5) + 0.01M NaCl
               (B): 0.01M TRis-HCl buffer(pH7.5) + 0.5M NaCl 
               Step gradient: 0min to 12min, (A) to (B)
Flow rate    : 0.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.