Agricultural Chemicals
The analyses of agricultural chemicals using two columns, RSpak DE-613 and GOLF-413 were performed. The analysis time can be shortened by using RSpak GOLF-413.
Sample :
1. Asulam, 2. Oxine-Cu, MCPP; 2. (Methyl-4-chlorophenoxy)-propionic acid
3. Thiuram, 4. Iprodione ,5. Bensulide, Pencycuron
Column : Shodex RSpak DE-613 (6.0mmID*150mm) Eluent : 50mM Sodium phosphate buffer(pH3.3)/CH3CN=49/51 Flow rate : 1.0mL/min Detector : UV(0min to 6.9min to 17.0min, 270nm to 243nm to 230nm) Column temp. : 40deg-C Column : Shodex RSpak GOLF-413 (4.6mmID*150mm) Eluent : 50mM Sodium phosphate buffer(pH3.2)/CH3CN=48/52 Flow rate : 1.0mL/min Detector : UV(0min to 3.5min to 5.0min, 270nm to 243nm to 230nm) Column temp. : 40deg-C
Thermally unstable and/or involatile agricultural chemicals which cannot be analyzed by GC or GC/MS can be analyzed by LC/MS.
A semi-micro column RSpak DE-213 was used for the separation.
Sample : 20ng each, 20micro-L
1. Asulam
2. 2-(Methyl-4-chlorophenoxy)-propionic acid, MCPP
3. Thiuram
4. Bensulide, SAP
5. Iprodione
6. Pencycuron
(HPLC)
Instrument : Agilent 1100
Column : Shodex RSpak DE-213 (2.0mmID*150mm)
Eluent : (A): 50mM CH3COONH4 aq.
(B): CH3CN
0min to 10min, (B)10% to (B)100%
Flow rate : 0.2mL/min
Column temp. : 40deg-C
(MS)
Instrument : Agilent 1100MSD
Mass range : 105-500(m/z)
Ionization : Electrospray
Polarity : Positive, Negative
Fragmentor : 60V
Nebulizer : N2(50psi)
Drying gas : N2(12L/min, 350deg-C)
Nine kinds of Agricultural Chemicals were analyzed by gradient elution with RSpak GOLF-413.
Sample : 20micro-L
| 1. |
10ppm |
Methyl 2-benzo imidazol carbamate, MBC |
| 2. |
50ppm
|
Asulam |
| 3. |
10ppm
|
Oxine-Cu |
| 4. |
40ppm
|
2-(Methyl-4-chlorophenoxy)-propionic acid, MCPP |
| 5. |
100ppm
|
Thiophanate-Methyl |
| 6. |
25ppm
|
Thiuram |
| 7. |
100ppm
|
Siduron |
| 8. |
50ppm
|
Iprodione |
| 9. |
100ppm
|
Bensulide, SAP |
Column : Shodex RSpak GOLF-413 (4.6mmID*150mm)
Eluent : (A): 50mM Potassium Phosphate buffer(pH3.0)/CH3CN=90/10, (B): CH3CN
Step gradient: 0min to 10min, 40%(B), 10min to 30min, 50%(B)
Flow rate : 1.0mL/min
Detector : Photodiode array(200nm-400nm max.plot)
Column temp. : 40deg-C
Oxine-copper easily makes coordination complex with other metals. When analyzed by an ODS column, the presence of metal-impurity in silica may cause adsorption and/or tailing. The packed gel used in golf course herbicides analysis column, RSpak GOLF-413 is a polymer base. The column does not contain metals thus prevents adsorption of oxine-copper.
Sample : Oxine-Cu, 20micro-L
|
GOLF-413
|
ODS
|
|
|
fas
|
1.152
|
2.042
|
Columns : Shodex RSpak GOLF-413,ODS(other manufacturer) (4.6mmID*150mm each) Eluent : 20mM H3PO4 aq./CH3CN=95/5 Flow rate : 1.0mL/min Detector : UV(254nm) Column temp. : 40deg-C