Column Test Conditions

Reversed Phase, Hydrophilic Interaction and Normal Phase Chromatography Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
ODP-50 6D ODP-50 6E ODP-50 4B ODP-50 4D ODP-50 4E ODP-50 2D	(1)0.75micro-L/mL n-Hexyl benzoate(ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E) 0.08% n-Hexyl Benzoate(ODP-50 2D) (2)10micro-L(ODP-50 6D), 17micro-L(ODP-50 6E), 3micro-L(ODP-50 4B), 6micro-L(ODP-50 4D, ODP-50 4E.), 7micro-L(ODP-50 2D), (3)CH₃CN/H₂O=65/35 (4)1.0mL/min(ODP-50 6D, ODP-50 6E), 0.6mL/min(ODP-50 4B, ODP-50 4D, ODP-50 4E), 0.2mL/min(ODP-50 2D) (5)30deg-C(ODP-50 6D, ODP-50 6E, ODP-50 4B, ODP-50 4D, ODP-50 4E), Ambient(ODP-50 2D) (6)UV(254nm)
ODP-40 4D ODP-40 4E	(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L (3)CH₃CN/H₂O=65/35 (4)0.6mL/min (5)30deg-C (6)UV(254nm)
C8P-50 4D C8P-50 4E	(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L(C8P-50 4D), 10micro-L(C8P-50 4E) (3)CH₃CN/H₂O=65/35 (4)0.6mL/min (5) 30deg-C (6)UV(254nm)
C4P-50 4D C4P-50 4E	(1)0.75micro-L/mL n-Hexyl benzoate (2)6micro-L (3)CH₃CN/H₂O=65/35 (4)0.6mL/min (5) 30deg-C (6)UV(254nm)
ODP2 HP-4B ODP2 HP-4D ODP2 HP-4E ODP2 HP-2B ODP2 HP-2D	(1)1,000mg/L Toluene (2)5micro-L(ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 2micro-L(ODP2 HP-2B), 1micro-L(ODP2 HP-2D) (3)H₂O/CH₃CN=55/45 (4)0.5mL/min(ODP2 HP-4B, ODP2 HP-4D, ODP2 HP-4E), 0.1mL/min(ODP2 HP-2B, ODP2 HP-2D) (5)40deg-C (6)UV(254nm)
RP18-415	(1)1% Acetone (2)3micro-L (3) CH₃CN/H₂O=95/5 (4)1.0mL/min (5)Ambient (6)UV(254nm)
RP18-613 RP18-413	(1)1% 3-Pentanone (2)5micro-L (3) CH₃CN/THF/H₂O=30/30/40 (4)0.8mL/min(RP18-613), 0.6mL/min(RP18-413) (5)Ambient (6)UV(262nm)
DS-613	(1)0.1% Benzene (2)20micro-L (3) CH₃CN/THF/H₂O=40/30/30 (4)0.8mL/min (5)Ambient (6)UV(254nm)
DS-413	(1)1% 3-Pentanone (2)5micro-L (3) CH₃CN/THF/H₂O=30/30/40 (4)0.6mL/min (5)Ambient (6)UV(262nm)
DE-613	(1)0.2% 1,5-Pentanediol (2)20micro-L (3) H₂O (4)0.8mL/min (5)Ambient (6)RI
DE-413 DE-413L DE-413S	(1)0.6% Di-n-butyl ketone (2)10micro-L(DE-413), 20micro-L(DE-413L), 8micro-L(DE-413S) (3) CH₃CN/H₂O=50/50 (4)1.0mL/min (5)40deg-C(DE-413, DE-413L), Ambient(DE-413S) (6)UV (262nm)
DE-213	(1)0.6% Di-n-butyl ketone (2)5micro-L (3) CH₃CN/H₂O=50/50 (4)0.2mL/min (5)40deg-C (6)UV (262nm)
GOLF-413 CARB-413 CARB-413L	(1)0.6% Di-n-butyl ketone (2)10micro-L (3) CH₃CN/H₂O=50/50 (4)1.0mL/min (5)40deg-C (6)UV (262nm)
DM-614	(1)2% Oxalic acid (2)5micro-L (3)5mM H₃PO₄ aq. (4)0.8mL/min (5)Ambient (6)RI
NN-814 NN-614 NN-414	(1)0.1% Uracil (2)10micro-L(NN-814), 7micro-L(NN-614, NN-414) (3)0.1M NaH₂PO₄(pH3.0) (4)1.0mL/min(NN-814), 0.8mL/min(NN-614), 0.3mL/min(NN-414) (5)35deg-C (6)UV(260nm)(NN-814, NN-614), RI(NN-414)
JJ-50 4D JJ-50 2D	(1)1.0% Acetone (2)2micro-L(JJ-4D), 0.5micro-L(JJ-2D) (3)CH₃CN/H₂O=60/40 (4)1.0mL/min(JJ-4D), 0.15mL/min(JJ-2D) (5)Ambient (6)UV(280nm)
NH2P-50 4D NH2P-50 4E NH2P-50 2D	(1)5mg/mL Sucrose (2)6micro-L(NH2P-50 4D, NH2P-50 4E), 1.5micro-L(NH2P-50 2D) (3)CH₃CN/H₂O=75/25 (4)0.6mL/min(NH2P-50 4D), 1.0mL/min(NH2P-50 4E), 0.2mL/min(NH2P-50 2D) (5)30deg-C(NH2P-50 4D, NH2P-50 4E), 35deg-C(NH2P-50 2D) (6)RI
F-411/S F-411 F-511	(1)0.15% t-Butyl benzene (2)20micro-L(F-411/S), 15micro-L(F-411, F-511) (3)CH₃OH/H₂O=80/20 (4)1.0mL/min (5)Ambient (6)UV(254nm)
C18M 4D C18M 4E C18P 4D C18P 4E	(1)0.61mg/mL Naphthalene (2)1.5micro-L(C18M 4D, C18P 4D), 2micro-L(C18M 4E, C18P 4E) (3)CH₃OH/H₂O=70/30 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
C18M 2D C18M 2E	(1)3.05mg/mL Naphthalene (2)0.2micro-L (3)CH₃CN/H₂O=60/40 (4)0.2mL/min (5)30deg-C (6)UV(254nm)
E-411
5SIL 4D 5SIL 4E	(1)0.5mg/mL p-Nitrobenzyl alcohol (2)1.5micro-L(5SIL 4D), 2micro-L(5SIL 4E) (3)Hexane/C₂H₅OH=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
5C8 4D 5C8 4E	(1)0.61mg/mL Naphthalene (2)1.5micro-L(5C8 4D), 2micro-L(5C8 4E) (3)CH₃OH/H₂O=66/34 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
5C4 4D	(1)0.61mg/mL Naphthalene (2)1.5micro-L (3)CH₃OH/H₂O=55/45 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
5CN 4D 5CN 4E	(1)0.60mg/mL Naphthalene (2)1.5micro-L(5CN 4D), 2micro-L(5CN 4E) (3)CH₃OH/H₂O=40/60 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
5NPE 4D	(1)0.61mg/mL Naphthalene (2)1.5micro-L (3)CH₃OH/H₂O=55/45 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
5NH 4D	(1)3.47mg/mL p-Nitrophenyl-α-D-glucopyranoside (2)1.5micro-L (3)CH₃CN/H₂O=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm)

Ion Exchange Chromatography Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
SP-825	(1)0.5% Acetone (2)20micro-L (3)50mM Na₂SO₄ aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
SP-420N	(1)0.2% Glycyl-L-tyrosine (2)1micro-L (3)20mM Acetate buffer + 0.5M Na₂SO₄(pH5.0) (4)1.0mL/min (5)Ambient (6)UV(280nm)
CM-825	(1)0.5% Acetone (2)20micro-L (3)50mM Na₂SO₄ aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
ES-502C 7C	(1)0.025% Cytosine (2)15micro-L (3)100mM Sodium phosphate buffer(pH4.4) (4)1.0mL/min (5)30deg-C (6)UV(250nm)
P-421S	(1)1% Ethylene glycol (2)4micro-L (3)H₂O (4)0.5mL/min (5)Ambient (6)RI
QA-825	(1)0.5% Acetone (2)20micro-L (3)50mM Na₂SO₄ aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
DEAE-825	(1)0.5% Acetone (2)20micro-L (3)50mM Na₂SO₄ aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
DEAE3N-4T	(1)200mg/L NaNO₃ (2)5micro-L (3)30mM phosphate buffer(pH6.0) + 0.2M NaCl (4)1.0mL/min (5)Ambient (6)UV(210nm)
ES-502N 7C	(1)0.025% Xanthine (2)10micro-L (3)50mM 1,3-Diaminopropane + 50mM NaCl(pH10.0) (4)1.0mL/min (5)30deg-C (6)UV(250nm)
WA-624	(1)0.03% UMP (2)10micro-L (3)0.1M Sodium phosphate buffer(pH3.0)/CH₃CN=80/20 (4)1.0mL/min (5)Ambient (6)UV(260nm)

Hydrophobic Interaction Chromatography Column

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
PH-814	(1)0.5% Acetone (2)20micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)UV(280nm)

Ion Chromatography Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
YS-50	(1)20mg/L Ca²⁺ (2)10micro-L (3)4mM Methanesulfonic acid aq. (4)1.0mL/min (5)40deg-C (6)CD
YK-421	(1)20mg/L Ca²⁺ (2)15micro-L (3)5mM Tartaric acid + 1mM Dipicolinic acid + 24mM Boric acid aq. (4)1.0mL/min (5)40deg-C (6)CD
Y-521	(1)20mg/L Na⁺ (2)50micro-L (3)4mM HNO₃ aq. (4)1.5mL/min (5)40deg-C (6)CD
T-521	(1)20mg/L Na⁺ (2)20micro-L (3)3mM HNO₃ aq. (4)1.0mL/min (5)40deg-C (6)CD
R-621	(1)1% Ethylene glycol (2)2micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
NI-424	(1)10mg/L SO₄^2- (2)50micro-L (3)8mM 4-Hydroxybenzoic acid + 2.8mM Bis(2-hydroxyethyl)imino- tris(hydroxymethyl)methane + 2mM Phenylboronic acid + 5micro-M trans-1,2-Aminocyclohexane-N,N,N’,N’-4 acetic acid aq.(4)1.0mL/min (5)40deg-C (6)CD
I-524A	(1)10mg/L NO₃^– (2)30micro-L (3)2.5mM Phthalic acid aq.(pH4.0) (4)1.5mL/min (5)40deg-C (6)CD
SI-90 4E	(1)15mg/L SO₄^2- (2)20micro-L (3)1.8mM Na₂CO₃ + 1.7mM NaHCO₃ aq. (4)1.0mL/min (5)Ambient (6)Suppressed CD
SI-91 4C	(1)10mg/L Br^– (2)20micro-L (3)1.8mM Na₂CO₃ + 1.7mM NaHCO₃ aq. (4)1.0mL/min (5)40deg-C (6)Suppressed CD
SI-50 4E	(1)10mg/L Br^– (2)20micro-L (3)3.2mM Na₂CO₃ + 1.0mM NaHCO₃ aq. (4)0.7mL/min (5)Ambient (6)Suppressed CD
SI-52 4E	(1)40mg/L SO₄^– (2)50micro-L (3)3.6mM Na₂CO₃ aq. (4)0.8mL/min (5)45deg-C (6)Suppressed CD

Chiral Separation Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
CDA-453 HQ	(1)0.01% 6-Fluoro-tryptophan (2)10micro-L (3)H₂O (4)0.3mL/min (5)20deg-C (6)UV(254nm)
CDB-453 HQ	(1)0.01% DL-Met β-naphthylamide (2)10micro-L (3)1.7% TEA/Acetic acid(pH4.0) + 0.1M NaCl in 20% CH₃CN + 80% H₂O (4)0.2mL/min (5)20deg-C (6)UV(254nm)
CDC-453 HQ	(1)0.02% Dancyl-leucine (2)20micro-L (3)1% Acetic acid + 0.2M NaCl in 10% CH₃CN + 90% H₂O (4)0.3mL/min (5)Ambient (6)UV(254nm)
CDBS-453	(1)0.1% Nefopam-HCl (2)10micro-L (3)CH₃CN/(1% Acetic acid + 0.2M NaCl) aq.=30/70 (4)0.5mL/min (5)Ambient (6)UV(254nm)
CRX-853	(1)0.1% RS-Mandelic acid (2)20micro-L (3)0.25mM CuSO₄ aq. (4)1.0mL/min (5)Ambient (6)UV(280nm)
ABA-894	(1)0.1% DL-Tryptophan (2)3micro-L (3)50mM Tris-HCl buffer + 0.15M(pH7.4) (4)1.0mL/min (5)Ambient (6)UV(280nm)

Ligand Exchange Chromatography Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
SC1011 SC1811	(1)1% Glycerol (2)5micro-L (3)H₂O (4)1.0mL/min (5)80deg-C (6)RI
SP0810	(1)1% Glycerol (2)5micro-L (3)H₂O (4)1.0mL/min (5)80deg-C (6)RI
KS-801 ～KS-806	(1)1% Ethylene glycol(KS-801), 2.5% Ethylene glycol(KS-802), 0.5% Ethylene glycol(KS-803), 0.8% Glucose(KS-804, KS-806), 0.4% Glucose(KS-805) (2)5micro-L(KS-801, KS-802, KS-804, KS-805, KS-806), 15micro-L(KS-803) (3)H₂O (4)1.0mL/min (5)50deg-C (6)RI
KS-807	(1)0.5% Glucose (2)5micro-L (3)H₂O (4)1.0mL/min (5)25deg-C (6)RI
SC1211	(1)1% Glycerol (2)10micro-L (3)CH₃CN/H₂O=25/75 (4)1.0mL/min (5)50deg-C (6)RI
DC-613	(1)2% Sucrose (2)8micro-L (3)CH₃CN/H₂O=70/30 (4)0.8mL/min (5)50deg-C (6)RI
SZ5532	(1)1% Glucose (2)5micro-L (3)CH₃CN/H₂O=70/30 (4)1.0mL/min (5)50deg-C (6)RI
MN-431	(1)0.5% Mannitol (2)10micro-L (3)H₂O (4)0.5mL/min (5)60deg-C (6)RI

Ion Exclusion Chromatography Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
SH1011 SH1821	(1)1% Glycerol (2)5micro-L (3)5mM H₂SO₄ aq. (4)1.0mL/min (5)50deg-C (6)RI
KC-811 KC-811 6E	(1)3% Acetic acid (2)5micro-L (3)0.1% H₃PO₄ aq. (4)1.0mL/min (5)40deg-C (6)RI

Size Exclusion Chromatography (SEC) Columns

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Column type	Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
KW-802.5 to KW-804	(1)0.2% Ethylene glycol (2)30micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
KW402.5 to KW405	(1)0.05% Uridine(KW402.5-4F, KW403-4F) 0.02% Uridine(2)1micro-L (3)50mM Phosphate buffer + 0.3M NaCl(pH7.0) (4)0.35mL/min (5)Ambient (6)UV(280nm)
SB-802 HQ to SB-806M HQ	(1)1% Ethylene glycol(802 HQ), 0.5% Ethylene glycol(802.5 HQ to 806M HQ) (2)10micro-L(802 HQ), 15micro-L(802.5 HQ to 806M HQ) (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
SB-807 HQ	(1)0.5% Glucose (2)10micro-L (3)H₂O (4)1.0mL/min (5)Ambient (6)RI
SB401 to SB404	(1)0.5% Ethylene glycol (2)5micro-L (3)H₂O (4)0.3mL/min (5)Ambient (6)RI
GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ	(1)1% Ethylene glycol (2)30micro-L (3)H₂O (4)0.6mL/min (5)30deg-C (6)RI
GS-320 4B GS-320 4D GS-320 2D	(1)0.5% Ethylene glycol (2)2micro-L(GS-320 4B, GS-320 4D), 1.5micro-L(GS-320 2D) (3)H₂O (4)0.25mL/min(GS-320 4B), 0.2mL/min(GS-320 4E), 0.05mL/min(GS-320 2D) (5)35deg-C (6)RI
GS-320 7E	(1)1% Ethylene glycol (2)25micro-L (3)H₂O (4)1.0mL/min (5)30deg-C (6)RI
GS-620 7G-P	(1)1% Ethylene glycol (2)50micro-L (3)H₂O (4)1.0mL/min (5)30deg-C (6)RI
GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ	(1)1% Ethylene glycol (2)30micro-L (3)H₂O (4)0.6mL/min (5)30deg-C (6)RI
GF-310 4B GF-310 4D GF-310 4E GF-310 2D	(1)0.5% Ethylene glycol (2)2micro-L(GF-310 4B, GF-310 4D), 3micro-L(GF-310 4E), 1micro-L(GF-310 2D) (3)H₂O (4)0.25mL/min(GF-310 4B), 0.2mL/min(GF-310 4D, GF-310 4E), 0.05mL/min(GF-310 2D) (5)35deg-C (6)RI
KF-801 to KF-807L	(1)0.1% Propylbenzene (2)20micro-L (3)THF (4)1.0mL/min (5)Ambient (6)UV(254nm)
K-801 to K-807L	(1)0.1% Propylbenzene (2)20micro-L (3)Chloroform (4)1.0mL/min (5)Ambient (6)UV(254nm)
KD-801 to KD-807	(1)1.0% Acetone (2)20micro-L (3)DMF (4)1.0mL/min (5)Ambient (6)RI
KF-601 to KF-607	(1)0.5% Propylbenzene(KF-601), 0.1% Dicyclohexyl phthalate(KF-602 to KF-607) (2)5micro-L (3)THF (4)0.5mL/min (5)Ambient (6)UV(254nm)
KF-401HQ to KF-406LHQ	(1)0.5% Propylbenzene(KF-401HQ), 0.1% Dicyclohexyl phthalate(KF-402HQ to KF-406LHQ) (2)5micro-L (3)THF (4)0.3mL/min (5)Ambient (6)UV(254nm)
HFIP-803 to HFIP-807	(1)5% Acetone (2)10micro-L (3)HFIP (4)1.0mL/min (5)Ambient (6)UV(280nm)
HFIP-603 to HFIP-607	(1)5% Acetone (2)2micro-L (3)HFIP (4)0.25mL/min (5)Ambient (6)UV(280nm)
HT-803 to HT-807	(1)10% Monochlorobenzene (2)20micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
UT-802.5 UT-806M UT-807	(1)10% Monochlorobenzene (2)10micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
AT-806MS	(1)1% Acetone (2)20micro-L (3)Toluene (4)1.0mL/min (5)Ambient (6)RI
LF-804 LF-604 LF-404	(1)0.1% Propylbenzene(LF-804), 0.1% Dicyclohexyl phthalate(LF-604, LF-404) (2)20micro-L(LF-804), 5micro-L(LF-604, LF-404) (3)THF (4)1.0mL/min(LF-804), 0.5mL/min(LF-604), 0.3mL/min(LF-404) (5)Ambient (6)UV(254nm)

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