DNA,RNA
DEAE-420N is an old type of DEAE3N-4T.
Sample : phi-X-174-RF DNA fragment
|
No.
|
base pairs
|
MW (x 105daltons)
|
|
1.
|
1353
|
8.79
|
|
2.
|
1078
|
7.01
|
|
3.
|
872
|
5.67
|
|
4.
|
603
|
3.92
|
|
5.
|
310
|
2.02
|
|
6B.
|
281
|
1.83
|
|
6A.
|
271
|
1.76
|
|
7.
|
234
|
1.52
|
|
8.
|
194
|
1.26
|
|
9.
|
118
|
0.77
|
|
10.
|
72
|
0.47
|
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Phosphate buffer(pH5.2)
(B); (A) + 1M NaCl
Linear gradient: 0min to 20min, 75% (B) to 100% (B)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30deg-C
IEC DEAE-825 packed with porous gel can allow larger sample loads than IEC DEAE3N-4T packed with non-porous gel.
Sample :
Transfer ribonucleic acid, tRNA (from E.Coli)
Column : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH6.6) + 250mM KCl + 5M Urea + 0.5mM EDTA
(B); (A) + 250mM KCl
Linear gradient: 0min to 150min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : Room temp.
Transfer ribonucleic acids were analyzed using OHpak SB-804 HQ (a column for GFC separation).
Please refer to Transfer RNA (3) (SB-805 HQ)
Sample :
1. 23s-RNA(E.Coli MRE 600)
2. 16s-RNA(E.Coli MRE 600)
3. 5s-RNA(E.Coli MRE 600)
4. Transfer ribonucleic acid, tRNA(E.Coli MRE 600)
Columns : Shodex OHpak SB-804 HQ (8.0mmID*300mm) x 2 Eluent : 0.1M CH3COONa buffer(pH7.0) + 0.3M NaCl + 1mM EDTA Flow rate : 0.1mL/min Detector : UV(260nm) Column temp. : Room temp.
Transfer ribonucleic acids were analyzed using OHpak SB-805 HQ (a column for GFC separation).
Refer to Transfer RNA (2) (SB-804 HQ)
Sample :
1. 23s-RNA(E.Coli MRE 600)
2. 16s-RNA(E.Coli MRE 600)
3. 5s-RNA(E.Coli MRE 600)
4. Transfer ribonucleic acid, tRNA(E.Coli MRE 600)
Columns : Shodex OHpak SB-805 HQ (8.0mmID*300mm) x 2 Eluent : 0.1M CH3COONa buffer(pH7.0) + 0.3M NaCl + 1mM EDTA Flow rate : 0.1mL/min Detector : UV(260nm) Column temp. : Room temp.
Transfer ribonucleic acids were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.
Sample : tRNA (E.Coli MRE600)
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Tris-HCl(pH7.5) (B); (A) + 1M NaCl
Linear gradient: 20%(B) to 50%(B), 0 to 20min
Flow rate : 0.5mL/min
Detector : UV(260nm)
Column temp. : 60deg-C
Ribosomel RNA is analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.
Sample : 5s-RNA (E.Coli MRE600)
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Tris-HCl buffer(pH7.5)
(B); (A) + 1M NaCl
Linear gradient: 0min to 15min, 10% (B) to 100% (B)
Flow rate : 1.5mL/min
Detector : UV(260nm)
Column temp : 60deg-C
Ribosomel RNA were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.
Sample : 16s+23s-RNA (E.Coli MRE600)
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Tris-HCl buffer(pH7.5)
(B); (A) + 1M NaCl
Linear gradient: 0min to 60min, 25% (B) to 100% (B)
Flow rate : 0.5mL/min
Detector : UV(260nm)
Column temp. : 30deg-C
Ribosomal RNA was analyzed using Asahipak GS-620 7G (a column for GFC separation). For general HPLC analysis, a high flow rate of 0.5-1.0mL/min or over is generally used. Whereas, for the GFC analysis of high molecular weight components, it is recommended that the analysis be made at a flow rate as low as possible, e.g. 0.1mL/min to prevent molecular chains from being cut off and to permit a high resolution.
GS-620 7G is an old type of GS-620 HQ.
Sample :
1. Ribosomal RNA-23S
2. Ribosomal RNA-16S
3. Ribosomal RNA-5S
Column : Shodex Asahipak GS-620 7G (7.5mmID*500mm) Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.3M NaCl Detector : UV(260nm) Column temp. : 30deg-C
Plasmid DNA fragments were analyzed using Asahipak GS-710 7G (a column for GFC separation).
GS-710 7G is an old type of GF-710 HQ.
Sample : Plasmid DNA Fragment
Columns : Shodex Asahipak GS-710 7G (7.5mmID*500mm) x 2 Eluent : 50mM Tris-HCl buffer(pH7.2) + 300mM NaCl + 2mM EDTA-2Na Flow rate : 0.1mL/min Detector : UV(260nm) Column temp. : 30deg-C
Crude gamma phage DNA fragments were analyzed using Asahipak GS-510 7G (a column for GFC separation).
GS-510 7G is an old type of GF-510 HQ.
Sample : Crude gamma phage DNA fragment
Column : Shodex Asahipak GS-510 7G (7.5mmID*500mm) Eluent : 0.1M Tris-HCl buffer(pH7.5) + 0.2M NaCl Flow rate : 0.6mL/min Detector : UV(260nm)
J. Hirabayashi and K. Kasai, Anal. Biochem., 178 (1989) 336.
Aqueous SEC column, OHpak SB-806 HQ was used for the analysis of polyadenylic acid.
Sample : 20micro-L
Polyadenylic acid, poly[A]n 0.1%
(Wako Pure Chemical Industries, Ltd.
code:101011 POLY-ADENYLIC ACID)
Column : Shodex OHpak SB-806 HQ (8.0mmID*300mm) Eluent : 10mM Sodium phosphate buffer(pH4.6) Flow rate : 1.0mL/min Detector : UV(260nm) Column temp. : 30deg-C