Hemoglobins were analyzed using IEC SP-420N ( a column for strong cation exchange chromatography packed with non-porous gel ).
Sample : Hemogrobin A, F, S and C
Column : Shodex IEC SP-420N (4.6mmID*35mm)
Eluent : (A); 20mM MES* buffer(pH5.6)
(B); (A) + 0.5M Na2SO4
Linear gradient: 0min to 30min, 5% (B) to 100% (B)
Flow rate : 1.5mL/min
Detector : VIS(415nm)
Column temp. : Room temp.
*MES : 2-(N-Morpholino) ethanesulfonic acid