GPC Clean-up of Phthalic Acid Esters
| EV | · Suitable for fractionation of residual pesticides in food · Used in the purification preocess of test solution preparation method in Shoku-Au No.1003001 (October 3ed, 2006. Japan) of thw Pharmaceutical and Food Safety Bureau, MHLW, Section 2 “Simultaneous GC/MS (LC/MS) Analysis of Agricultual Chemicals in Livestock and Marine Products” |
| PAE | · Suitable for cleaning up water samlpes such as blood and bottom sediment · Highly effective for Fractionation of endocrine disruptors in environmental samples |
(GPC clean-up for residual pesticides in food, etc)
|
Product code
|
Product name
|
Particle size
(micron) |
Size(mm)
ID x Length |
|---|---|---|---|
| F6090005 | CLNpak EV-200 | 16 | 2.0 x 150 |
| F6090001 F6090002 |
CLNpak EV-2000 CLNpak EV-G |
16 16 |
20.0 x 300 20.0 x 100 |
| F6090003 F6090004 |
CLNpak EV-2000 AC CLNpak EV-G AC |
16 16 |
20.0 x 300 20.0 x 100 |
| Product name |
Max. sample load (mL) |
Max. usable pressure (MPa) |
Recommended flow rate (mL/min) |
Shipping
solvent |
|---|---|---|---|---|
| EV-200 |
0.2
|
2.5 | 0.05 |
Ethylacetate/Cyclohexane=3/7
|
| EV-2000 EV-G |
5
– |
2.5 – |
4.0 to 5.0 – |
Ethylacetate/Cyclohexane=3/7
Ethylacetate/Cyclohexane=3/7 |
| EV-2000 AC EV-G AC |
5
– |
2.5 – |
4.0 to 5.0 – |
Acetone/Cyclohexane=3/7
Acetone/Cyclohexane=3/7 |
Usable eluent : Acetone/Cyclohexan=1/9 to 5/5, Ethylacetate/Cyclohexane=1/9 to 5/5
(GPC clean-up for phthalic acid esters in sediments, bioogical samples, blood, etc)
|
Product code
|
Product name
|
Particle size
(micron) |
Size(mm)
ID x Length |
|---|---|---|---|
| F7600025 F6810022 F6714007 |
CLNpak PAE-800 CLNpak PAE-2000 CLNpak PAE-G |
5 5 9 |
8.0 x 300 20.0 x 300 8.0 x 50 |
| F7600026 F6810023 F6714008 |
CLNpak PAE-800 AC CLNpak PAE-2000 AC CLNpak PAE-G AC |
5 5 9 |
8.0 x 300 20.0 x 300 8.0 x 50 |
| Product name |
Max. sample load(mL) |
Max. usable pressure (MPa) |
Recommended flow rate (mL/min) |
Shipping
solvent |
|---|---|---|---|---|
| PAE-800 PAE-2000 PAE-G |
0.5
3 – |
4.0 5.0 – |
0.8 3.5 – |
Acetonitrile
Acetonitrile Acetonitrile |
| PAE-800 AC PAE-2000 AC PAE-G AC |
0.5 (water:10%)
3 (water:10%) – |
4.0 5.0 – |
0.8 3.5 – |
Acetone
Acetone Acetone |
Usable eluent : Acetone, Acetonitrile
CLNpak PAE-800 was used for GPC clean-up of phthalic acid esters. Compared with the case of analysis with acetone eluent, the saparation of peak 1 and peak 3 to 14 is insufficient.
Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
3-14. Phthalic acid esters
14. Dimethyl phthalate
15. H2O
16. CO2
17. Sulfur
Column : Shodex CLNpak PAE-800 (8.0mmID*300mm) Eluent : Acetonitrile Flow rate : 0.5mL/min Column temp. : Room temp.
CLNpak PAE-2000 was used for GPC clean-up of phthalic acid esters. Please compare with the case of analysis using PAE-800 and analysis with acetone eluent.
Sample : 1mL, dissolved in acetonitrile
1. Corn oil
2. Butylbenzyl phthalate
3. H2O
4. Sulfur
5-16. Phthalic acid esters
5. Di-ethylhexyl phthalate, 6. Di-n-octyl phthalate, 7. Di-heptyl phthalate, 8. Di-n-hexyl phthalate,
9. Di-pentyhl phthalate, 10. Di-isobutyl phthalate, 11. Di-n-butyl phthalate, 12. Di-n-propyl phthalate,
13. Di-cyclohexyl phthalate, 14. Butylbenzyl phthalate, 15. Di-ethyl phthalate, 16. Di-methyl phthalate
Columns : Shodex CLNpak PAE-G (8.0mmID*50mm) + PAE-2000 (20.0mmID*300mm) Eluent : Acetonitrile Flow rate : 4.0mL/min Detector : UV(254nm),RI Column temp. : 40deg-C
CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters. Compared with the case of analysis with acetonitrile eluent, the saparation of peak 1 and peak 3 to 14 is better.
Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
3-14. Phthalic acid esters
14. Dimethyl phthalate
15. Sulfur
16. H2O
17. CO2
Column : Shodex CLNpak PAE-800 AC (8.0mmID*300mm) Eluent : Acetone Flow rate : 0.5mL/min Column temp. : Room temp.
CLNpak PAE-2000 AC was used for GPC clean-up of phthalic acid esters. Please compare with the case of analysis using PAE-800 AC and analysis with acetonitorile eluent.
Sample : 1mL, dissolved in acetone
1. Corn oil
2. Butylbenzyl phthalate
3. Sulfur
4. H2O
5-16. Phthalic acid esters
5. Di-ethylhexyl phthalate, 6. Di-n-octyl phthalate, 7. Di-heptyl phthalate, 8. Di-n-hexyl phthalate,
9. Di-pentyhl phthalate, 10. Di-isobutyl phthalate, 11. Di-n-butyl phthalate, 12. Di-n-propyl phthalate,
13. Di-cyclohexyl phthalate, 14. Butylbenzyl phthalate, 15. Di-ethyl phthalate, 16. Di-methyl phthalate
17. beta-carotene
18. Acetonitorile
Columns : Shodex CLNpak PAE-G AC (8.0mmID*50mm) + PAE-2000 AC (20.0mmID*300mm) Eluent : Acetone Flow rate : 4.0mL/min Column temp. : 40deg-C
CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters. Using marker samples having UV absoption, the position of fractionating phthalic acid esters can be determined.
Sample :
1. Corn oil
2. Bis(2-ethylhexyl) adipate
3. Di-2-ethylhexyl phthalate
4. Dimethyl phthalate
5. beta-carotene (dissolved in acetone)
6. Acetonitrile
Column : Shodex CLNpak PAE-800 AC (8.0mmID*300mm) Eluent : Acetone Flow rate : 0.5mL/min Column temp. : Room temp.
CLNpak PAE-800 AC was used for GPC clean-up of phthalic acid esters.
Sample :
1. Corn oil
2. Sulfur
3. H2O
4. CO2
5. Bis(2-ethylhexyl) adipate
6. Phthalic acid esters
7. 4-Nonylphenol
8. DDT
9. Bisphenol A
10. Antracene
Column : Shodex CLNpak PAE-800 AC (8.0mmID*300mm) Eluent : Acetone Flow rate : 0.5mL/min Detector : UV(330nm), RI Column temp. : Room temp.
Two CLNpak PAE columns which have different column size are compared. PAE-2000 (20mmID x 300mm) column has 6.25 times larger cross section area than PAE-800 (8mmID x 300mm) column and large amount of sample can be loaded to PAE-800 column.
Sample :
1. 0.090mg/500micro-L Corn oil
2. 0.036mg/500micro-L Di-2-ethylhexyl phthalate
3. 0.540mg/3000micro-L Corn oil
4. 0.216mg/3000micro-L Di-2-ethylhexyl phthalate
Columns : Shodex CLNpak PAE-800 AC (8.0mmID*300mm) Eluent : Acetone Flow rate : 0.5mL/min Column temp. : Room temp. |
: PAE-2000 AC (20.0mmID*300mm) : Acetone : 3.4mL/min : Room temp. |