IEC DEAE-420N is suited for fast analysis as it is packed in a small column (4.6mmID x 35mm) and packed with non-porous gel bonded with diethylaminoethyl groups.
DEAE-420N is an old type of DEAE3N-4T.
Sample : Oligoadenylic acid, poly[A]n
Column : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent : (A); 25mM Phosphate buffer(pH6.0)
(B); (A) + 1M NaCl(pH6.0)
Linear gradient: 0min to 30min, 10% (B) to 100% (B)
Flow rate : 1.5mL/min
Detector : UV(260nm)
Column temp. : Room temp.