Amino acids were analyzed using IC YS-50 for cations. YS-50 is packed with polyvinyl alcohol with carboxyl functional group. Amino acids are separated by reversed-phase mode and ionic interaction, as NN-814 column does Alanine and Glycine elute at the same elution time under this condition.
See also Amino Acids (2) (NN-814).
Sample : 0.02% each, 5micro-L
1. Aspartic acid
Column : Shodex IC YS-50 (4.6mmID*125mm)
Eluent : 2mM Acetic acid aq.
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 40deg-C