Comparison of Sample Retention Using ODP, C8P and C4P Columns

The difference of retention of three columns for reversed phase chromatography, Asahipak ODP, C8P and C4P were compared.

Sample :
1. beta-Endorphin
2. Big gastrin
3. Insulin

Columns      : Shodex Asahipak ODP-50, C8P-50, C4P-50 (4.6mmID*150mm each)
Eluent       : 0.05% TFA aq./CH3CN=72/28
Flow rate    : 0.6mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C