Purine Bases and Pyrimidine Bases (1) (GS-320 HQ)

Purine bases and pyrimidine bases were analyzed using Asahipak GS-320 HQ (a multimode column).

Sample : 50micro-g/mL each, 20micro-L
1. Cytosine
2. Adenine
3. Guanine
4. Uracil
5. Hypoxanthine
6. Thymine

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 50mM NaH2PO4 aq./50mM H3PO4 aq.=500/380
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C