Calibration Curves
A column, which has the most appropriated calibration curve for the sample molecular weight range should be selected. For example, regarding the two columns (1) and (2), whose calibration curves are shown in the figure, column (2) is best suited for the analysis of the mixture of A, B and C. In other words, among the column calibration curves which covers all the sample molecular weight range, a column whose calibration curve has the smallest inclination should be selected to obtain a good separation.
| THF Eluent | Generally, polystyrene (PS) standards are used as calibration standards for organic SEC (GPC) with THF eluent. However, when there is a large difference between the structure of the polymer sample and that of the polystyrene, other standards may be used, if the structure is close to that of the polymer. Poly(ethylene glycol) (PEG) or poly(methyl methacrylate) (PMMA) can be used for this purpose. |
| Chloroform Eluent | PS standards are commonly used for organic SEC (GPC) analysis with chloroform eluent. |
| DMF Eluent | Polyethylene oxide (PEO) and PEG are used for the calibration standards of organic SEC (GPC) analysis with DMF eluent, when using KD-800 columns. If PS is used for the standard, PS elutes slower because PS is adsorbed by the packing material of KD-800 columns. Different from KD-800 columns, PS as well as PEG and PEO can be used as calibration standards for the SB-800 HQ series with DMF eluent. For samples, which cannot be analyzed by the KD-800 columns because of the adsorption by the packing material, the SB-800 HQ series can be employed. |
| Toluene Eluent | PS is used for the standard for organic SEC (GPC) analysis with toluene eluent. |
| Quinoline Eluent | PEG is used for the standard for organic SEC (GPC) analysis with guinoline eluent. |
| Methanol Eluent | PEO and PEG is used for the standard for organic SEC (GPC) analysis with methanol eluent because they are easily dissolved in methanol. |
| Aqueous Eluent | Pullulan, PEO and PEG are used for Aqueous SEC (GFC) analysis with aqueous eluents, such as pure water or aqueous salt solution. |
Calibration curves for GPC K-800 series are shown.
Sample : PS, Polystyrene
100micro-L each
Columns : Shodex GPC K-800 series (8.0mmID*300mm each) Eluent : CHCl3 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for GPC K-800L series, linear type columns are shown.
Sample : PS, Polystyrene
Columns : Shodex GPC K-800L series (8.0mmID*300mm each) Eluent : CHCl3
Calibration curves for GPC KF-800 series are shown.
Sample : PS, Polystyrene
Columns : Shodex GPC KF-800 series (8.0mmID*300mm each) Eluent : THF
Calibration curves for GPC KF-800L series, linear type columns are shown.
Sample : PS, Polystyrene
Columns : Shodex GPC KF-800L series (8.0mmID*300mm each) Eluent : THF
GPC KF-806M and KF-806L are both packed with mixed type gel. However, compared with KF-806M, KF-806L which was developed recently has better linearity in the calibration curve.
Sample : PS, Polystyrene
Columns : Shodex GPC KF-806M, KF-806L (8.0mmID*300mm each) Eluent : THF
Around the range of molecular weight 100, a solvent peak and/or other peaks coming from polymer additives may appear. For improving the separation in the low molecular weight range without affecting the separation in the high molecular weight range, it is recommended to use a solvent-peak separation column such as GPC KF-800D, KF-600D, KF-400D (for THF) or K-800D (for chloroform). The solvent-peak separation column should be used with linear type columns.
Sample : PS, Polystyrene
Calibration curves for GPC KF-600 series are shown.
Sample : PS, Polystyrene
Column : Shodex GPC KF-600 series (6.0mmID*150mm each) Eluent : THF
These are calibration curves of GPC KF-401HQ to KF-404HQ.
Sample : PS, Polystyrene
Columns : Shodex GPC KF-401HQ , KF-402HQ, KF-403HQ, KF-404HQ (4.6mmID*250mm each) Eluent : THF Flow rate : 0.3mL/min
These are calibration curves of GPC KF-405LHQ andKF-406LHQ.
Sample : PS, Polystyrene
Columns : Shodex GPC KF-405LHQ x 2, KF-406LHQ x 2 (4.6mmID*250mm each) Eluent : THF Flow rate : 0.3mL/min Column temp. : 40deg-C
Calibration curves are shown for the GPC KF-806L with the following three different eluents. The difference of the calibration curves can be explained by the difference of molecular sizes of polystyrene. The three dimensional size of polystyrene is considered to be different depending on the kind of solvent in which the polystyrene is dissolved.
Sample : PS, Polystyrene
Column : Shodex GPC KF-806L (8.0mmID*300mm) Eluent : 1; DMF, 2; DMAc, 3; NMP Column temp. : 40deg-C
DMF: Dimethylformamide
DMAc: Dimethylacetamide
NMP: N-Methylpyrrolidone
Calibration curves for GPC LF-804 is shown. The good linear line can be obtained in the range of molecular weights 300-2,000,000.
Sample : PS, Polystyrene
Columns : Shodex GPC LF-804 (8.0mmID*300mm) x 3 Eluent : THF Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40deg-C
Different kinds of pullulan were separated using organic SEC (GPC) column GPC LF-804. DMSO/DMF eluent is sometimes necessary to analyse starch. In this case, linear type LF-804 is effective.
Sample : 100micro-L
Pullulan (MW:1,600,000) 0.05%
Pullulan (MW:788000) 0.1%
Pullulan (MW:404,000) 0.1%
Pullulan (MW:212,000) 0.1%
Pullulan (MW:112,000) 0.1%
Pullulan (MW:47,300) 0.1%
Pullulan (MW:22,800) 0.1%
Pullulan (MW:11,800) 0.1%
Pullulan (MW:5,900) 0.1%
Maltotriose (MW:504) 0.1%
Maltose (MW:342) 0.1%
Glucose (MW:180) 0.1%
Column : Shodex GPC LF-804 (8.0mmID*300mm) Eluent : 10mM LiBr in DMSO/DMF=75/25 Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 50deg-C
The calibration curve of PEG/PEO standards using organic SEC (GPC) column LF-804 with DMAc as an eluent is shown.
Sample : 200micro-L
PEO, Poly(ethylene oxide)
PEG, Poly(ethylene glycol)
Columns : Shodex GPC LF-804 (8.0mmID*300mm) x 2 Eluent : 30mM LiBr in DMAc Flow rate : 0.7mL/min Detector : Shodex RI Column temp. : 40deg-C
The calibration curve of polystyrene standards using organic SEC (GPC) column GPC LF-804 with DMAc as an eluent is shown.
Sample : 200micro-L
PS, Polystyrene
Columns : Shodex GPC LF-804 (8.0mmID*300mm) x 2 Eluent : 30mM LiBr in DMAc Flow rate : 0.7mL/min Detector : Shodex RI Column temp. : 40deg-C
Poly(methyl methacrylate) (PMMA) standards were analyzed using organic SEC (GPC) column GPC LF-804.
Sample : Poly(methyl methacrylate) (PMMA)
100micro-L
(MW: 1,520,000) 0.05%
(MW: 772,000) 0.1%
(MW: 211,000) 0.1%
(MW:55,900) 0.1%
(MW:21,700) 0.1%
(MW:6,900) 0.1%
(MW:2,220) 0.1%
Columns : Shodex GPC LF-804 (8.0mmID*300mm) x 2 Eluent : Methyl ethyl ketone Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40deg-C
GPC LF-404 and LF-604 are downsized columns of LF-804. Comparison of calibration curves for GPC LF-404 and LF-604 are shown. They show good linear lines as same as LF-804.
Sample : PS, Polystyrene
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2, Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : THF Flow rate : 0.3mL/min(LF-404), 0.6mL/min(LF-604) Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Calibration curve for GPC LF-604 is shown.
Sample : 20micro-L
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : DMF Flow rate : 0.5mL/min Detector : Shodex RI(small cell volume) Column temp. : 50deg-C
Calibration curve for GPC LF-604 is shown.
Sample : 20micro-L
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : NMP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp.: 50deg-C
Calibration curve for GPC LF-604 is shown.
Sample : PS, Polystyrene, 20micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : NMP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp.: 50deg-C
Calibration curves for GPC KD-800 series are shown.
Sample : PEO, Poly(ethylene oxide)
Columns : Shodex GPC KD-800 series (8.0mmID*300mm) Eluent : DMF
Calibration curves for GPC HFIP-800 series are shown.
Sample :
PMMA, Poly(methyl methacrylate)
Ethyl methacrylate
Columns : Shodex GPC HFIP-800 series (8.0mmID*300mm each) Eluent : HFIP Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : Room temp.
Calibration curves for HFIP-600 series are shown.
Sample : PMMA, Poly(methyl methacrylate)
Columns : Shodex GPC HFIP-600 series (6.0mmID*150mm) Eluent : 5mM CF3COONa in HFIP Detctor : Shodex RI(small cell volume) Column temp. : 40deg-C
Calibration curves for GPC KD-800 series are shown.
Sample : PEO, Poly(ethylene oxide)
Columns : Shodex GPC KD-800 series (8.0mmID*300mm) Eluent : DMF
Calibration curves for GPC HFIP-800 series are shown.
Sample :
PMMA, Poly(methyl methacrylate)
Ethyl methacrylate
Columns : Shodex GPC HFIP-800 series (8.0mmID*300mm each) Eluent : HFIP Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : Room temp.
Calibration curves for PROTEIN KW-800 series (columns for aqueous SEC (GFC)) are shown.
Sample :
1. Thyroglobulin (MW: 669,000)
2. Aldolase (MW: 158,000)
3. BSA (MW: 67,000)
4. Ovalbumin (MW: 43,000)
5. Peroxidase (MW: 40,200)
6. Adenylate kinase (MW: 32,000)
7. Myoglobin (MW: 17,000)
8. Ribonuclease A (MW: 13,700)
9. Aprotinin (MW: 6,500)
10. Vitamin B12 (MW: 1,350)
Columns : Shodex PROTEIN KW-800 series (8.0mmID*300mm each) Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : Room temp.
Calibration curves for PROTEIN KW-802.5 (a column for Aqueous SEC (GFC)) are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Proteins
Column : Shodex PROTEIN KW-802.5 (8.0mmID*300mm)
Eluent : (Proteins); 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
(Pullulan, PEG, PEO); H2O
Flow rate : 1.0mL/min
Detector : (Proteins); UV(220nm), (Pullulan, PEG, PEO); Shodex RI
Column temp. : Room temp.
Calibration curves for PROTEIN KW-803 (a column for Aqueous SEC (GFC)) are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Proteins
Column : Shodex PROTEIN KW-803 (8.0mmID*300mm)
Eluent : (Proteins); 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
(Pullulan, PEG, PEO); H2O
Flow rate : 1.0mL/min
Detector : (Proteins); UV(220nm), (Pullulan, PEG, PEO); Shodex RI
Column temp. : Room temp.
Calibration curves for PROTEIN KW-804 (a column for Aqueous SEC (GFC)) are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Proteins
Column : Shodex PROTEIN KW-804 (8.0mmID*300mm)
Eluent : (Proteins); 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
(Pullulan, PEG, PEO); H2O
Flow rate : 1.0mL/min
Detector : (Proteins); UV(220nm), (Pullulan, PEG, PEO); Shodex RI
Column temp. : Room temp.
KW400-4F series is aqueous SEC (GFC) columns and downsized KW-800 series. Figure is calibration curves of KW400-4F series columns with pullulan. KW405-4F is newly line-upped column, and it was designed having larger pore size than KW-804. KW405-4F can analyze high molecular weight substances more than 1,000,000 as protein like agglomerated protein. KW400-4F series can be applied wider range of molecular weight substances than KW-800 series.
Sample : Pullulan
Columns : Shodex KW400-4F series (4.6mmID*300mm each) Eluent : H2O Flow rate : 0.33mL/min Detector : Shodex RI (small cell volume) Column temp. : Room temp.
KW400-4F series is aqueous SEC (GFC) columns and downsized KW-800 series. Figure is calibration curves of KW400-4F series columns with pullulan. KW405-4F newly line-upped column, and it was designed having larger pore size than KW-804. KW405-4F can analyze high molecular weight substances more than 1,000,000 as protein like agglomerated protein. KW400-4F series can be applied wider range of molecular weight substances than KW-800 series.
Sample : Proteins
Columns : Shodex KW400-4F series (4.6mmID*300mm each) Eluent : 50mM Sodium phosphate buffer (pH7.0) + 0.3M NaCl Flow rate : 0.33mL/min Detector : UV(280nm) Column temp. : 25deg-C
KW400-4F series is aqueous SEC (GFC) columns and downsized KW-800 series. Figure is calibration curves of KW400-4F series columns with pullulan. KW405-4F newly line-upped column, and it was designed having larger pore size than KW-804. KW405-4F can analyze high molecular weight substances more than 1,000,000 as protein like agglomerated protein. KW400-4F series can be applied wider range of molecular weight substances than KW-800 series.
Sample :
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex KW400-4F series (4.6mmID*300mm each) Eluent : H2O Flow rate : 0.33mL/min Detector : Shodex RI Column temp. : 25deg-C
Calibration curves for OHpak SB-800 HQ series are shown.
Sample :
Pullulan
Maltotriose
Maltose
Glucose
Columns : Shodex OHpak SB-800 HQ series (8.0mmID*300mm each) Eluent : H2O Detector : Shodex RI Column temp. : Room temp.
Calibration curves for OHpak SB-800 HQ series are shown.
Sample :
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex OHpak SB-800 HQ series (8.0mmID*300mm each) Eluent : H2O
Calibration curves for OHpak SB-800 HQ series are shown.
Sample : PS, Polystyrene
Columns : Shodex OHpak SB-800 HQ series (8.0mmID*300mm each) Eluent : DMF
Calibration curves for OHpak SB-800 HQ series are shown.
Sample :
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : SHodex OHpak SB-800 HQ series (8.0mmID*300mm each) Eluent : 20mM LiBr in DMF Flow rate : 0.8mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for OHpak SB-800 HQ series are shown.
Sample : PMMA, Poly(methyl methacrylate)
Columns : Shodex OHpak SB-800 HQ series (8.0mmID*300mm each) Eluent : 20mM LiBr in DMF Flow rate : 0.8mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for OHpak SB-806M HQ are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex OHpak SB-G + SB-806M HQ (8.0mmID*300mm) x 2 Eluent : 50mM NaNO3 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 35deg-C
Calibration curves for OHpak SB-806M HQ are shown. For samples, which cannot be analyzed using the GPC KD-800 columns because of the adsorption by the packing material, the OHpak SB-800 HQ columns can be employed.
However, frequent replacement of in-column eluent is not recommended as it may shorten column life.
Sample :
PEO, Poly(ethylene oxide)
PEG, Poly(ethylene glycol)
PS, Polystyrene
Column : Shodex OHpak SB-806M HQ (8.0mmID*300mm) Eluent : DMF Column temp. : 40deg-C
SB400-4E series is aqueous SEC (GFC) type columns and downsized SB-800 HQ series. Figure is calibration curves of SB400-4E series columns with pullulan. SB401-4E is newly line-upped column, and it was designed having smaller pore size than SB-802 HQ.
Sample : Pullulan
Maltotriose
Maltose
Glucose
Columns : Shodex SB400-4E series (4.6mmID*300mm each) Eluent : H2O Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : Room temp.
Calibration curves for GPC KD-800 series and OHpak SB-800 HQseries are shown. Compared with the KD-800 columns, the SB-800 HQ columns perform better over a low molecular weight range.
Sample :
PEO, Poly(ethylene oxide)
PEG, Poly(ethylene glycol)
Columns : Shodex OHpak SB-805 HQ + SB-802.5 HQ (8.0mmID*300mm each)
Shodex GPC KD-805 + KD-802.5 (8.0mmID*300mm each)
Eluent : DMF
Column temp. : 40deg-C
For the calibration curve for GPC KD-802.5 , PEG and PEO should be used as the standards. The calibration curve with PS is far different from that with PEG and PEO. For the calibration curve for OHpak SB-802.5 HQ, both PEG, PEO and PS can be used as the standards. The calibataion cuve with PS is almost as same as that with PEG and PEO.
Sample :
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
PS, Polystyrene
Columns : Shodex GPC KD-802.5, OHpak SB-802.5 HQ (8.0mmID*300mm each) Eluent : DMF Column temp. : 40deg-C
Calibration curves for Asahipak GS-HQ series are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
Columns : Shodex Asahipak GS-HQ series (7.5mmID*300mm each) Eluent : H2O Flow rate : 0.6mL/min Detector : RI Column temp. : 30deg-C
A calibration curve for Asahipak GS-520 7G (a multimode column) is shown. When an eluent with a relatively high salt concentration is used for Asahipak GS series, substances are eluted in decreasing order of molecular weight as it is the GFC mode which function mainly. GS-520, which has a large pore size, can analyze proteins with molecular weight up to 1,000,000. Samples such as human and bovine serum albumins which exhibit strong affinity with the molecular structure of the packing material are eluted belatedly. Making use of this characteristics, GS-520 is used exclusively for the analysis of serum albumin.
GS-520 7G is an old type of GS-520 HQ.
Sample :
1. IgM
2. IgA
3. IgG
4. Transferrin
5. BSA
6. Ovalbumin
7. Chymotrypsinogen A
8. Myoglobin
9. Ribonuclease
10. Cytochrome c
Column : Shodex Asahipak GS-520 7G (7.5mmID*500mm) Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.3M NaCl Flow rate : 1.0mL/min Detector : UV(210nm) Column temp. : Room temp.
Calibration curves for Asahipak GF-510 HQ and GF-7M HQ (columns for GFC separation) are shown.
Sample:
1. IgM
2. IgA
3. IgG
4. Transferrin
5. Conalbumin
6. BSA
7. Ovalbumin
8. Myoglobin
9. Ribonuclease A
10. Cytochrome c
11. L-Phenylalanin
12. Creatinine
Columns : Shodex Asahipak GF-510 HQ (7.5mmID*300mm) : GF-7M HQ (7.5mmID*300mm) Eluent : 0.1M Sodium phosphate buffer(pH7.0) + 0.1M NaCl : 0.2M Sodium phosphate buffer(pH7.0) Flow rate : 0.5mL/min : 0.5mL/min Detector : UV(280nm) : UV(280nm) Column temp. : 30deg-C : 30deg-C
Calibration curves for Asahipak GF-HQ series are shown.
Sample : PS, Polystyrene
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : CHCl3 Flow rate : 0.6mL/min Detector : UV(254nm) Column temp. : 30deg-C
Calibration curves for Asahipak GF-HQ series are shown.
Sample :
Pullulan
PEG, Poly(ethylene glycol)
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : H2O Flow rate : 0.6mL/min Detector : RI Column temp. : 30deg-C
Calibration curves for Asahipak GF-HQ seires are shown.
Sample : PS, Polystyrene
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm) Eluent : 20mM LiBr in DMF Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for Asahipak GF-HQ series are shown.
Sample :
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : 20mM LiBr in DMF Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for Asahipak GF-HQ series are shown.
Sample : PMMA, Poly(methyl methacrylate)
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : 20mM LiBr in DMF Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
Calibration curves for Asahipak GF-HQ series are shown using acetone eluent for PMMA.
Sample :
PMMA, Poly(methylmethacrylate) (MW:772,000)
PMMA, Poly(methyl methacrylate) (MW:211,000)
PMMA, Poly(methyl methacrylate) (MW:55,900)
PMMA, Poly(methyl methacrylate) (MW:21,700)
PMMA, Poly(methyl methacrylate) (MW:6,900)
PMMA, Poly(methyl methacrylate) (MW:2,220)
Column : Shodex Asahipak GF-310 HQ, GF-510 HQ, GF-710 HQ, GF-7M HQ (7.5mmID*300mm each) Eluent : Acetone Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
A calibration curve for Asahipak GF-510 HQ + GF-310 HQ is shown.
Sample : PS, Polystyrene
Column : Shodex Asahipak GF-510 HQ + GF-310 HQ (7.5mmID*300mm each) Eluent : THF Flow rate : 0.5mL/min Detetctor : UV(254nm) Column temp. : 30deg-C
A calibration curve for Asahipak GF-510 HQ + GF-310 HQ is shown.
Sample : PS, Polystyrene
Column : Shodex Asahipak GF-510 HQ + GF-310 HQ (7.5mmID*300mm each) Eluent : DMF Flow rate : 0.5mL/min Detetctor : UV(270nm) Column temp. : 30deg-C
A calibration curve for Asahipak GF-710 HQ is shown.
Sample :
1. PEO, Poly(ethylene oxide) (MW: 860,000)
2. PEO, Poly(ethylene oxide) (MW: 160,000)
3. PEO, Poly(ethylene oxide) (MW: 21,000)
4. PEG, Poly(ethylene glycol) (MW: 4,000)
5. PEG, Poly(ethylene glycol) (MW: 300)
6. EG, Ethylene glycol (MW: 62)
Column : Shodex Asahipak GF-710 HQ (7.5mmID*300mm) Eluent : 50mM LiCl in CH3OH Column temp. : 30deg-C
Calibration curves for GPC UT-800 sereis are shown.
Sample : PS, Polystyrene
Columns : Shodex GPC UT-800 series (8.0mmID*300mm each) Eluent : THF Flow rate : 1.0mL/min Detecto : Shodex RI Column temp. : Room temp.
Since Asahipak GF-HQ series can be used with both aqueous and organic solvents, they are suited to the analysis of hydrophilic polymers such as sodium poly(styrene sulfonate) and sodium polyacrylate. Several kinds of pydrophilic polymer standard are commercially available. When analyzing a sample, a standard which resembles it in structure should be selected to obtain the calibration curve for the analysis.
Sample : Sodium poly(styrene sulfonate) standard
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : 50mM LiCl aq./CH3CN=60/40 Flow rate : 0.4mL/min Detector : Shodex RI Column temp. : 30deg-C
Since Asahipak GF-HQ series can be used with both aqueous and organic solvents, they are suited to the analysis of hydrophilic polymers such as sodium poly(styrene sulfonate) and sodium polyacrylate. Several kinds of pydrophilic polymer standard are commercially available. When analyzing a sample, a standard which resembles it in structure should be selected to obtain the calibration curve for the analysis.
Sample : Sodium polyacrylate standard
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : 50mM Na2HPO4 aq./CH3CN=90/10 Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
Since Asahipak GF-HQ series can be used with both aqueous and organic solvents, they are suited to the analysis of hydrophilic polymers such as sodium poly(styrene sulfonate) and sodium polyacrylate. Several kinds of pydrophilic polymer standard are commercially available. When analyzing a sample, a standard which resembles it in structure should be selected to obtain the calibration curve for the analysis.
Sample : Poly(sodium methacrylate) standard
Columns : Shodex Asahipak GF-HQ series (7.5mmID*300mm each) Eluent : 50mM Na2HPO4 aq./CH3CN=90/10 Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 40deg-C
The calibration curves for downsized organic SEC (GPC) columns using PMMA are shown. In the case of the analysis for polymer whose MW is 1,000,000 or less, GPC LF-604 or LF-404 is suitable. When it is needed to separate low MW polymer and high MW polymer and low MW polymer is the purpose for the analysis, GPC HFIP-603 is suitable.
Please refer to Comparison of Analysis of PMMA with Down-sized SEC Columns
Sample : PMMA, Poly(methyl methacrylate) standards, 50micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2
Shodex GPC LF-404 (4.6mmID*250mm) x 2
Shodex GPC KF-405LHQ (4.6mmID*250mm) x 2
Shodex GPC KF-406LHQ (4.6mmID*250mm) x 2
Shodex GPC HFIP-603 (6.0mmID*150mm) x 2
Shodex GPC HFIP-606M (6.0mmID*150mm) x 2
Eluent : 5mM CF3COONa in HFIP
Flow rate : 0.3mL/min
Detector : Shodex RI(small cell volume)
Column temp. : 40deg-C