Features of RSpak DE Series (7) (Effect of Sample Load on Column Efficiency)

Di-n-butyl ketone were analyzed using RSpak DE-413 (a column for reversed phase chromatography). When the sample concentration was constant, a decrease of column efficiency was observed accroding to an icrease of injection volume. The decrease started from 50micro-L and it was marked when the injection volume exceeded 100micro-L. To the contrary, when the injection volume was constant, a decrease of column efficiency was not observed until sample load exceeded 500micro-g.

Sample : Di-n-butyl ketone

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : CH3CN/H2O=50/50
Flow rate    : 1.0mL/min
Detector     : UV(262nm)
Column temp. : 40deg-C