Basic Peptides

Basic peptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).

Sample :
1. Sub.P Frg.1-4
2. Glu-Gly-Phe
3. Neuro.Frg.8-13
4. Met-Enkephalin
5. alpha-Endorphin
6. Des-Arg⁹-Bradykinin
7. Substance P

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 0.05% TFA aq./CH3CN=80/20
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C