Effect of Sample Load on Peak Width (DEAE-420N)

Effect of sample load on peak width for IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel) is shown.
DEAE-420N is an old type of DEAE3N-4T.

Sample : Ovalbumin

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 25mM Piperazine-HCl(pH6.0)
               (B); (A) + 1M NaCl
               Linear gradient: 0min to 20min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.