Standard Proteins (21) (SP-420N)

Six kinds of protein standards were analyzed using IEC SP-420N (a column for strong cation exchange chromatography packed with non-porous gel).

Sample :
1. Myoglobin
2. Trypsinogen
3. Ribonuclease A
4. alpha Chymotrypsinogen A
5. Cytochrome c
6. Lysozyme

Column       : Shodex IEC SP-420N (4.6mmID*35mm)
Eluent       : (A); 20mM Sodium phosphate buffer(pH7.0)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 10min, (A) to (B)
Flow rate    : 1.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.