Fast Analysis of Hexokinase

Hexokinase was analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel). Fast analysis is possible using DEAE-420N compared with using IEC DEAE-825 (a column for weak anion exchange chromatography packed with porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample :
Hexokinase

               (Left)                                        (Right)            
Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)          : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 20mM Tris-HCl buffer(pH7.0)            : (A); 25mM Tris-HCl buffer(pH8.0)
               (B);(A) + 0.5M NaCl                           (B);(A) + 0.5M NaCl 
                Linear gradient: 0min to 60min, (A) to (B)   Linear gradient: 0min to 10min, (A) to (B)
Flow rate    : 1.0mL/min                                   : 1.5mL/min
Detector     : UV(280nm)                                   : UV(280nm)
Column temp. : Room temp.                                  : Room temp.