Effect of Salt Concentration

Effect of salt concentration was measured using HIC PH-814 ( a hydrophobic interaction column ).

Sample :
1. alpha Chymotrypsinogen A
2. alpha Chymotrypsin
3. Lysozyme
4. Ovalbumin
5. Ribonuclease A
6. Myoglobin
7. Cytochrome c

Column    : Shodex HIC PH-814 (8.0mmID*75mm)

Eluent    : (A): (B) + Ammonium sulfate

            (B):  0.1M Phosphate buffer(pH7.0)

            Linear gradient: (A) to (B)

Flow rate : 1.0mL/min