Human IgM

Human IgM was analyzed using AFpak APR-894 (an affinity column).

Sample : Human IgM (Sigma)

Column       : Shodex AFpak APR-894 (8.0mmID*50mm)
Eluent       : (A); 0.02M Sodium phosphate buffer(pH6.5)
               (B); (A) + 1.0M NaCl
               Linear gradient: (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.