Dansyl Amino Acids
Dansyl-amino acid was analyzed using ODSpak F-411 (a column for reversed phase chromatography) and a chemiluminescence detectot (CL) and a fluorescence detector detector compared.
Sample :
1.Dansyl-L-Valine
2.Dansyl-L-Proline
Column : Shodex ODSpak F-411 (4.6mmID*150mm) Eluent : 0.1M Imidazole Buffer(pH7.0, adjusted by nitrate)/CH3CN=25/75 Flow rate : 0.5mL/min Reagent : [1mM TCPO in Ethyl acetate}/[0.15M H2O2(30%aq) in CH3CN]=25/75 Detector : CL, Fluorescence detector(Ex. 330nm, Em. 560nm) Column temp. : 30deg-C