Purine Bases and Pyrimidine Bases (4) (DE-413)

Six components were completely separated using RSpak DE-413 (a column for reversed phase chromatography).

Sample : 0.01% each, 5micro-L
1. Cytosine
2. Uracil
3. Hypoxanthine
4. Guanine
5. Thymine
6. Adenine

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : 0.05M KH2PO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 25deg-C