Adenosine and Derivatives

Adenosine and derivatives were analyzed using Asahipak GS-320 HQ (a multimode column).

Sample : 100micro-g/mL each, 20micro-L
1. ATP
2. ADP
3. AMP
4. Adenine
5. Adenosine

Column       : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent       : 200mM NaH2PO4 aq./ 200mM H3PO4 aq.=500/62 
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C