Nucleotides (8) (DEAE-825)

Nucleotides were analyzed using IEC DEAE-825 (a column for weak anion exchange chromatography).

Sample :
1. CMP
2. AMP
3. UMP
4. GMP
5. CDP
6. ADP
7. UDP
8. CTP
9. GDP
10. ATP
11. UTP
12. GTP

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 0.1M NaH2PO4 aq./CH3CN=80/20
               (B); 0.5M NaH2PO4 aq./CH3CN=80/20
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 25deg-C