Multimode and Aqueous/Organic SEC Columns
Column Specifications
| GS-HQ | · SEC mode is the main mode · Multimode columns for reversed phase, HILIC, and ion exchange modes are available depending on selected eluent conditions · Suitable for the separation od peptodes or nucleic acids with similar molecular weights · Suitable for desalting samples or substituting buffer in protein analysis |
| GS-320 7E | · For the analysis of nucleic acids as “Umami” and its metabolite with isocratic elution |
| GS-620 7G-P | · Column to determine mean molecular weight distribution of gelatin compliant with PAGI method (ver. 10, Japan) |
| MSpak GS-320 | · Semi-micro columns for Asahipak GS-320 HQ · Effective fot the analysis of chemical substances in a biological sampel by column switching intended to remove proteins · Applicable to analysis using LC/MS or ICP-MS |
(Standard Columns)
| Product code | Product name | Plate number (TPN/column) |
Exclusion limit (Pullulan) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|---|
| F7600005 F7600006 F7600007 F7600008 F6710019 |
Asahipak GS-220 HQ Asahipak GS-320 HQ Asahipak GS-520 HQ Asahipak GS-620 HQ Asahipak GS-2G 7B |
> 19,000
> 19,000 > 18,000 > 18,000 Guard column |
*(3,000) 40,000 300,000 **(2,000,000) – |
6 6 7 7 9 |
7.5 x 300 7.5 x 300 7.5 x 300 7.5 x 300 7.5 x 50 |
| Product name |
Max. usable pressure (MPa) |
Recommended flow rate (mL/min) |
Max. usable flow rate (mL/min) |
Max. usable temp. (deg-C) |
Usable pH range |
Shipping solvent |
|---|---|---|---|---|---|---|
| GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ GS-2G 7B |
6.0
5.0 3.0 3.5 – |
0.4 to 0.6
0.4 to 0.6 0.4 to 0.6 0.4 to 0.6 – |
1.0
1.0 1.0 1.0 – |
60
60 60 60 50 |
2 to 9
2 to 12 2 to 12 2 to 12 2 to 12 |
CH3OH/H2O=30/70
CH3OH/H2O=30/70 CH3OH/H2O=30/70 CH3OH/H2O=30/70 CH3OH/H2O=30/70 |
*( ): PEG euivalent
**( ): Estimated value
Max. usable organic solvent concentration:
GS-320 HQ, GS-520 HQ, GS-620 HQ : 100%(Methanol), 50%(Acetonitrile)
GS-220 HQ : 30%(Methanol), 50%(Acetonitrile)
For the analysis of nucleic acids as “Umami”
|
Product code
|
Product name | Scope of application | Size(mm) ID x Length |
Shipping solvent |
|---|---|---|---|---|
| Asahipak GS-320 7E Asahipak GS-2G 7B |
Umami” (nucleic acids) (Guard column) |
7.5 x 250 7.5 x 50 |
CH3OH/H2O=30/70
CH3OH/H2O=30/70 |
For determination of the molecular weight distribution og gelatin
|
Product code
|
Product name | Scope of application | Size(mm) ID x Length |
Shipping solvent |
|---|---|---|---|---|
| Asahipak GS-620 7G-P Asaipak GS-2G 7B |
Gelatin for photo film (Guard column) |
7.5 x 500 7.5 x 50 |
CH3OH/H2O=30/70
CH3OH/H2O=30/70 |
(Simi-micro columns)
|
Product code
|
Product name | Plate number (TPN/column) |
Particle size (micron) |
Size(mm) ID x Length |
Shipping solvent |
|---|---|---|---|---|---|
| MSpak GS-320 4B MSpak GS-320 4D MSpak GS-320 2D |
> 2,000
> 7,000 > 4,000 |
6 6 6 |
4.6 x 50 4.6 x 150 2.0 x 150 |
H2O
H2O H2O |
(Micro column and Semimicro columns)(Remark)
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GS220A-4E GS220A-4D GS220A-4B GS220A-2E GS220A-2D GS220A-2B GS220A-1E GS220A-1D GS220A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GS-220 HQ
|
|
| GS220A-M8E GS220A-M8D GS220A-M8B GS220A-M5E GS220A-M5D GS220A-M5B GS220A-M3E GS220A-M3D GS220A-M3B |
0.8×250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GS-220 HQ
|
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GS320A-4E GS320A-4D GS320A-4B GS320A-2E GS320A-2D GS320A-2B GS320A-1E GS320A-1D GS320A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GS-320 HQ
|
|
| GS320A-M8E GS320A-M8D GS320A-M8B GS320A-M5E GS320A-M5D GS320A-M5B GS320A-M3E GS320A-M3D GS320A-M3B |
0.8×250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GS-320 HQ
|
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GS520A-4E GS520A-4D GS520A-4B GS520A-2E GS520A-2D GS520A-2B GS520A-1E GS520A-1D GS520A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GS-520 HQ
|
|
| GS520A-M8E GS520A-M8D GS520A-M8B GS520A-M5E GS520A-M5D GS520A-M5B GS520A-M3E GS520A-M3D GS520A-M3B |
0.8×250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GS-520 HQ
|
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GS620A-4E GS620A-4D GS620A-4B GS620A-2E GS620A-2D GS620A-2B GS620A-1E GS620A-1D GS620A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GS-620 HQ
|
|
| GS620A-M8E GS620A-M8D GS620A-M8B GS620A-M5E GS620A-M5D GS620A-M5B GS620A-M3E GS620A-M3D GS620A-M3B |
0.8×250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GS-620 HQ
|
(Preparative Columns)(Remark)
| Product code | Product name | Plate number (TPN/column) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|
| Asahipak GS-220 20F Asahipak GS-320 20F Asahipak GS-520 20F Asahipak GS-620 20F Asahipak GS-220 20G Asahipak GS-320 20G Asahipak GS-520 20G Asahipak GS-620 20G Asahipak GS-20G 7B |
> 8,000
> 8,000 > 8,000 > 8,000 > 14,000 > 14,000 > 14,000 > 14,000 Guard column |
13
13 13 13 13 13 13 13 20 |
20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 7.5 x 50 |
| Product name | Flow rate (mL/min) | Standard column |
|
|---|---|---|---|
| Usual | Max. | ||
| GS-220 20F GS-320 20F GS-520 20F GS-620 20F GS-220 20G GS-320 20G GS-520 20G GS-620 20G GS-20G 7B |
4 to 6 5 to 8 4 to 6 4 to 6 4 to 6 5 to 8 4 to 6 4 to 6 – |
8 12 8 8 8 12 8 8 – |
GS-220 HQ
GS-320 HQ GS-520 HQ GS-620 HQ GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ Guard column |
(Older Columns)(Remark)
| Product code | Product name | Size(mm) ID x Length |
Recommended
column |
|---|---|---|---|
| Asahipak GS-220 7G Asahipak GS-320 7G Asahipak GS-520 7G Asahipak GS-620 7G Asahipak GS-220 7E Asahipak GS-320 7E Asahipak GS-520 7E Asahipak GS-620 7E Asahipak GS-2G 7B |
7.5 x 500
7.5 x 500 7.5 x 500 7.5 x 500 7.5 x 250 7.5 x 250 7.5 x 250 7.5 x 250 7.5 x 50 |
GS-220 HQ
GS-320 HQ GS-520 HQ GS-620 HQ GS-220 HQ GS-320 HQ GS-520 HQ GS-620 HQ Guard column |
| GF-HQ | · Polymer-based SEC column exhibits solvent durability · Supports both aqueous and organic solvents |
| GF-310 | · Semi-micro columns for Asahipak GF-310 HQ · Effective fot the analysis of chemical substances in biological samples by column switching intending to remove proteins · Useful for LC/MS analysis · Suitable for the analysis of surfactants |
(Standard Column)
| Product code | Product name | Plate number (TPN/column) |
Exclusion limit (Pullulan) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|---|
| F7600001 F7600002 F7600003 F7600004 F6710018 |
Asahipak GF-310 HQ Asahipak GF-510 HQ Asahipak GF-710 HQ Asahipak GF-7M HQ Asahipak GF-1G 7B |
> 19,000
> 19,000 > 11,000 > 13,000 Guard column |
40,000 300,000 *(10,000,000) *(10,000,000) – |
5 5 9 9 9 |
7.5 x 300 7.5 x 300 7.5 x 300 7.5 x 300 7.5 x 50 |
| Product name |
Max. usable pressure (MPa) |
Recommended flow rate (mL/min) |
Max. usable flow rate (mL/min) |
Max. usable temp. (deg-C) |
Usable pH range |
Shipping solvent |
|---|---|---|---|---|---|---|
| GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ GF-1G 7B |
7.0
6.5 2.5 4.5 – |
0.4 to 0.6
0.4 to 0.6 0.4 to 0.6 0.4 to 0.6 – |
1.0
1.0 1.0 1.0 – |
60
60 60 60 60 |
2 to 9
2 to 9 2 to 9 2 to 9 2 to 9 |
CH3OH/H2O=30/70
CH3OH/H2O=30/70 CH3OH/H2O=30/70 CH3OH/H2O=30/70 CH3OH/H2O=30/70 |
*( ): Estimated values
| Usable solvents | ||||||||
|---|---|---|---|---|---|---|---|---|
| Water with salt |
0 to 0.5M
|
Yes
|
Acetone |
0 to 100%
|
Yes
|
|||
| Methanol |
0 to 100%
|
Yes
|
Chloroform |
0 to 100%
|
Yes
|
|||
| Ethanol |
0 to 100%
|
Yes
|
Ethyl acetate |
0 to 100%
|
Yes
|
|||
| Acetonitrile |
0 to 100%
|
Yes
|
DMSO |
0 to 50%
|
Yes
|
|||
| THF |
0 to 100%
|
Yes
|
DMSO |
51 to 100%
|
No
|
|||
| DMF |
0 to 100%
|
Yes
|
||||||
(Semi-micro columns)
| Product code | Product name | Plate number (TPN/column) |
Particle size (micron) |
Size(mm) ID x Length |
Shipping solvent |
|---|---|---|---|---|---|
| MSpak GF-310 4B MSpak GF-310 4D MSpak GF-310 4E MSpak GF-310 2D |
> 3,000
> 10,000 > 16,000 > 5,500 |
5 5 5 5 |
4.6 x 50 4.6 x 150 4.6 x 250 2.0 x150 |
H2O
H2O H2O H2O |
(Micro column and Semimicro columns)(Remark)
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GF310A-4E GF310A-4D GF310A-4B GF310A-2E GF310A-2D GF310A-2B GF310A-1E GF310A-1D GF310A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GF-310 HQ
|
|
| GF310A-M8E GF310A-M8D GF310A-M8B GF310A-M5E GF310A-M5D GF310A-M5B GF310A-M3E GF310A-M3D GF310A-M3B |
0.8 x 250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GF-310 HQ
|
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GF510A-4E GF510A-4D GF510A-4B GF510A-2E GF510A-2D GF510A-2B GF510A-1E GF510A-1D GF510A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GF-510 HQ
|
|
| GF510A-M8E GF510A-M8D GF510A-M8B GF510A-M5E GF510A-M5D GF510A-M5B GF510A-M3E GF510A-M3D GF510A-M3B |
0.8 x 250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GF-510 HQ
|
|
Product code |
Product name |
Size(mm) ID x Length |
Standard column |
|---|---|---|---|
| GF710A-4E GF710A-4D GF710A-4B GF710A-2E GF710A-2D GF710A-2B GF710A-1E GF710A-1D GF710A-1B |
4.6 x 250
4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50 |
GF-710 HQ
|
|
| GF710A-M8E GF710A-M8D GF710A-M8B GF710A-M5E GF710A-M5D GF710A-M5B GF710A-M3E GF710A-M3D GF710A-M3B |
0.8 x 250
0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50 |
GF-710 HQ
|
(Preparative Columns)(Remark)
|
Product code |
Product name | Plate number (TPN/column) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|
| Asahipak GS-310 20F Asahipak GS-510 20F Asahipak GS-710 20F Asahipak GSM-700 20F Asahipak GS-310 20G Asahipak GS-510 20G Asahipak GS-710 20G Asahipak GSM-700 20G Asahipak GS-10G 7B |
> 8,000
> 8,000 > 8,000 > 8,000 > 14,000 > 14,000 > 14,000 > 14,000 Guard column |
13
13 13 13 13 13 13 13 20 |
20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 7.5 x 50 |
| Product name | Flow rate (mL/min) | Standard column |
|
|---|---|---|---|
| Usual | Max. | ||
| GS-310 20F GS-510 20F GS-710 20F GSM-700 20F GS-310 20G GS-510 20G GS-710 20G GSM-700 20G GS-10G 7B |
5 to 8 5 to 8 2 to 3 2 to 3 5 to 8 5 to 8 2 to 3 2 to 3 – |
12 12 4 4 12 12 4 4 – |
GF-310 HQ
GF-510 HQ GF-710 HQ GF-7M HQ GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ Guard column |
(Older Columns)(Remark)
|
Product code |
Product name | Size(mm) ID x Length |
Recommended column |
|---|---|---|---|
| Asahipak GS-310 7G Asahipak GS-510 7G Asahipak GS-710 7G Asahipak GSM-700 7G Asahipak GS-310 7E Asahipak GS-510 7E Asahipak GS-710 7E Asahipak GSM-700 7E Asahipak GF-1G 7B |
7.5 x 500 7.5 x 500 7.5 x 500 7.5 x 500 7.5 x 250 7.5 x 250 7.5 x 250 7.5 x 250 7.5 x 50 |
GF-310 HQ
GF-510 HQ GF-710 HQ GF-7M HQ GF-310 HQ GF-510 HQ GF-710 HQ GF-7M HQ GF-1G 7B |
Column Information
Although Asahipak GS-HQ series is basically SEC columns, they permit different types of separation and analysis from conventional ones by use of a multimode which combines the hydrophobic interaction (RPC) and ionic interaction (IEX: ion exclusion). The GS-320 HQ column performs well in separating hydrophillic peptides, particularly acidic and basic peptides. As the columns can be used over a wide range of eluent pH because of being polymer-based column, they can be washed with alkaline solution leading to a longer column life, which is another feature of the column.
Sample : 20micro-L
1. Glu-Ala-Glu 0.025%
2. Arg-Asp 0.05%
3. Gly-His-Lys 0.025%
4. Arg-Pro-Lys-Pro 0.025%
| MW | pI | Σf | |
|---|---|---|---|
| Glu-Ala-Glu |
347
|
3.12
|
0.39
|
| Arg-Asp |
289
|
6.75
|
0.68
|
| Gly-His-Lys |
340
|
9.95
|
0.29
|
| Arg-Pro-Lys-Pro |
497
|
11.44
|
3.24
|
pI : Isoelectric point
Σf : Hydrophobic parameter
Column : Shodex Asahipak GS-320 HQ (7.5mmID*300mm) Eluent : 50mM Ammonium acetate buffer(pH6.7) Flow rate : 0.5mL/min Detector : UV(220nm) Column temp. : 30deg-C
1) When a neutral/acidic eluent is used.
| Acidic samples | As it is the ion exclusion mode which is in operation, substances are eluted relatively quickly. |
| Neutral samples | As substances are separated by reversed phase mode, they are eluted moderately. |
| Basic samples | As it is the ion exchange mode which is in operation, substances are eluted relatively slow. |
2) When a basic eluent is used.
| Acidic samples | Basically, substances are excluded together at the Vo position but, with acidic samples of large molecular weights, there are cases in which substances are retained in the column. |
| Neutral samples | Substances are separated by reversed phase mode. |
| Basic samples | If the eluent has a higher pH than the pKa of the sample, substances are separated by reversed phase mode as the dissociation of basic samples is suppressed. |
As the eluent for Asahipak GS-HQ series and GF-HQ series, either organic solvent or aqueous solution can be used.
Organic solvents: Please refer to Multimode Columns and Aqueous/Organic SEC Columns.
Aqueous solutions: Please refer to the followings:
1. Strong electrolytic salts
Total salts concentration should be 0.5 M or less. Aqueous solutions of NaCl, KCl, Na2SO4, K2SO4, (NH4)2SO4 and other such salts may be used as eluents. Phosphoric acid, acetic acid, citric acid, boric acid, and tris-HCI buffer solutions may also be used, either independently or in combination with these salts.
2. Aqueous uric acid and guanidine hydrochloride
These solutions are widely used as protein denaturalization agents. As their concentration is generally high, it is recommended that they be used in columns set aside exclusively for this purpose, as their frequent interchange with eluents of lower concentration would tend to shorten the column service life.
3. Use of surfactants
Aqueous solutions containing SDS, Brij-35 or other surfactants can also be used as eluents. The eluent interchange time following the use of such an eluent will generally be longer than for ordinary eluents. Approx. 50% methanol may be used to shorten the replacement time.
4. Solutions with high salt content
After passage of a high-concentration salt solution, its removal from the flow lines by flushing with water is sometimes necessary to prevent salt precipitation at the pump and the injector. In such cases, column life is best preserved by first removing the column, before flushing the LC system flow lines.