Bisphenol A

Bisphenol A which is one of endocrine disruptors was analyzed using RSpak DE-413. The detection limit was 3.8ng.

Sample : 0.05mg/mL Bisphenol A, 5micro-L

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : H2O/CH3CN=50/50
Flow rate    : 1.0mL/min
Detector     : UV(217nm)
Column temp. : 40deg-C

CLNpak PAE-2000 can be used for the clean-up of bisphenol A.
Please compare with the case of Clean-up of Bisphenol A (PAE-2000 AC).

Sample : 1mL
1. Butter
2. H2O
3. p-tert-butylphenol
4. Phenol
5. Bisphenol A

Columns      : Shodex CLNpak PAE-G (8.0mmID*50mm) + PAE-2000 (20.0mmID*300mm)
Eluent       : Acetonitrile
Flow rate    : 4.0 mL/min
Detector     : UV(254nm), RI
Column temp. : 40deg-C

CLNpak PAE-2000 AC can be used for the clean-up of bisphenol A.
Please compare with the case of Clean-up of Bisphenol A (PAE-2000).

Sample : 1mL or 10micro-L, dissolved in acetone
1. Butter
2. H2O
3. p-tert-butylphenol
4. Phenol
5. Bisphenol A

Column       : Shodex CLNpak PAE-G AC (8.0mmID*50mm) + PAE-2000 AC (20.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 4.0 mL/min
Detector     : UV(254nm), RI
Column temp. : 40deg-C

Polycyclic Aromatic hydrocarbons (PAHs) such as Benzo[a]pyrene existing in the air are known as the carcinogen or mutagen. Recentry, it developed that Nitro-polycyclic aromatic hydrocarbon (NPAH), which in one of carcinogen, is in also existing in the air. And the carcinogens and mutagens come from diesel dust. The following application data shows that the separation of carcinogens existing in the diesel dust with using GPC clean up column, CLNpak PAE-800 AC column.

Sample :
1. Bisphenol A
2. Benzo[a]fluoranthene
3. 1,3-Dinitropyrene
4. Benzo[a]pyrene
5. Hydroxybenzo[a]pyrene mixture

Column       : Shodex CLNpak PAE-800 AC (8.0mmID*300mm)
Eluent       : Acetone
Flow rate    : 0.8mL/min
Detector     : UV(210nm)
Column temp. : Room temp.

Courtesy of Dr. Kazuichi Hayakawa, Faculty of Pharmaceutical Science, Kanazawa University