BSA Analysis in Surfactant (On-line Surfactant Removal)

Surfactants are often used to solubilize cell membrane and/or to prevent peptide/protein to be adsorbed on the container surface. However, the presence of surfactant may deteriorate LC column and LC/MS system, results in poor repeatability. By using column-switching sample preparation column, MSpak PK or GF, removes surfactants prior to sample being introduced to the analytical column. Therefore provides good repeatable analysis and reduces the time required for sample preparation.

Sample: BSA

Pretreatment
Column       : Shodex MSpak GF-4A (4.6mmID*10mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 150mM NaCl
Flow rate    : 1.0mL/min
Column temp. : Room temp.
Switching time : 5min
Analysis 
Column       : Shodex PROTEIN KW-804 + KW-802.5 (8.0mmID*300mm each)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 150mM NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.