Guidelines for Shodex Column Selection : Vitamins, Hormones and Lipidsadmin2021-12-22T19:50:23+00:00
Guidelines for Shodex Column Selection : Vitamins, Hormones and Lipids
In the past, vitamins were analyzed by bioassay or enzyme assay. However, these methods were complicated and differed according to the kind of vitamins to be analyzed, it was impossible to analyze several kinds of vitamins at one time.
Recently, HPLC method as well as the above two methods has been adopted as one of the official methods in Japan. And then, HPLC analysis using reversed phase mode or normal phase mode is widely accepted for vitamin analysis.
Hormones of Mammalia can be classified into peptides, amino acids, steroids, catecholamines, etc. Most columns used in the section of “Proteins, Peptides and Amino Acids” also can be used for the analysis of hormones.
Reversed phase mode analysis is especially suitable for the analysis of steroids and catecholamines.
Reversed phase mode and normal phase mode are used for the analysis of lipids. Here, not only the above modes but also GFC mode and affinity chromatography mode are explained.