Linear Type Columns: LF-404, 604
| LF | · Employs a special packing materials with a wide pore size distribution (multi pore type) · Enables analysis over a broad range of molecular weights (100 to2 million) · Highly linear calibration curve without infection points · Molecular weight distribution can be determined with high precision · Column for rapid analysis (LF-604) and columns for high performance analysis (LF-404) enabling reduction in solvent use are also available |
Please refer to Replacement of In-Column Solvent
*Use of the LF-604 and the LF-404 with semi-micro type devices is recommended.
(Standard column) shipping solvent : Tetrahydrofulan (THF)
| Product code | Product name | Plate number (TPN/column) |
Exclusion limit
(Polystyrene) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|---|
|
GPC LF-804
GPC LF-G |
> 17,000
Guard column |
2,000,000
– |
6
6 |
8.0 x 300 4.6 x 10 |
|
Product
name |
Max. usable pressure(MPa) |
Recommended
flow rate(mL/min) |
Max. usable flow rate(mL/min) |
Max. usable temp. (deg-C) |
|---|---|---|---|---|
|
LF-804
LF-G |
3.5
– |
1.0
– |
2.0
– |
20 to 60 20 to 60 |
(Rapid analysis downsized column) shipping solvent : Tetrahydrofulan (THF)
| Product code | Product name | Plate number (TPN/column) |
Exclusion limit
(Polystyrene) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|---|
|
GPC LF-604
GPC LF-G |
> 9,000
Guard column |
2,000,000
– |
6
6 |
6.0 x 150 4.6 x 10 |
|
Product
name |
Max. usable pressure(MPa) |
Recommended
flow rate(mL/min) |
Max. usable flow rate(mL/min) |
Max. usable temp. (deg-C) |
|---|---|---|---|---|
|
LF-604
LF-G |
3.5
– |
0.5
– |
1.0
– |
20 to 60 20 to 60 |
(High-perfomance semi-micro column) shipping solvent : Tetrahydrofulan (THF)
| Product code | Product name | Plate number (TPN/column) |
Exclusion limit
(Polystyrene) |
Particle size (micron) |
Size(mm) ID x Length |
|---|---|---|---|---|---|
|
GPC LF-404
GPC LF-G |
> 14,000
Guard column |
2,000,000
– |
6
6 |
4.0 x 250 4.6 x 10 |
|
Product
name |
Max. usable pressure(MPa) |
Recommended
flow rate(mL/min) |
Max. usable flow rate(mL/min) |
Max. usable temp. (deg-C) |
|---|---|---|---|---|
|
LF-404
LF-G |
3.5
– |
0.3
– |
0.6
– |
20 to 60 20 to 60 |
GPC LF-404 and LF-604 are downsized columns of LF-804. Comparison of calibration curves for GPC LF-404 and LF-604 are shown. They show good linear lines as same as LF-804.
Sample : PS, Polystyrene
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2, Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : THF Flow rate : 0.3mL/min(LF-404), 0.6mL/min(LF-604) Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Comparison of chromatograms of GPC LF-404, LF-604 and LF-804 are shown. LF-404 shows as same as LF-804. LF-604 gives rapid separation.
Sample : PS, Polystyrene
Columns : Shodex GPC LF-804 (8.0mmID*300mm) x 2
Shodex GPC LF-404 (4.6mmID*250mm) x 2
Shodex GPC LF-604 (6.0mmID*150mm) x 2
Eluent : THF
Flow rate : (LF-804); 1.0mL/min, (LF-404); 0.3mL/min, (LF-604); 0.6mL/min
Detector : Shodex RI (LF-804; conventional, LF-404,604; small cell volume)
Column temp. : 40deg-C
Please refer to the following tables for the classification of solvents in terms of capability of being replaced with original in-column solvent. However, frequent replacement of in-column solvent may shorten column life, and is, therefore, not recommended. We can replace the in-column solvent with another solvent such as o-dichlorobenzene, ethyl acetate, dimethylacetamide or methylpyrrolidon upon your request.
(Standard Organic SEC (GPC) columns)
(Downsized GPC columns and Semi-micro GPC columns)
(Linear Type : LF series)
Calibration curve for GPC LF-604 is shown.
Sample : 20micro-L
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : DMF Flow rate : 0.5mL/min Detector : Shodex RI(small cell volume) Column temp. : 50deg-C
Calibration curve for GPC LF-604 is shown.
Sample : 20micro-L
PEG, Poly(ethylene glycol)
PEO, Poly(ethylene oxide)
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : NMP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp.: 50deg-C
Calibration curve for GPC LF-604 is shown.
Sample : PS, Polystyrene, 20micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : NMP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp.: 50deg-C
Each one, two and three units of GPC LF-404, were used for the analysis of the mixture of standard polystyrene compounds. Only a single unit of LF-404 can determine a broad range of molecular weights from 300 to 2,000,000. However the resolution is higher depending on the number of columns, so it is better to use several units of columns for obtaining more accurate molecular weight distribution.
Sample : 10micro-L
1. PS, Polystyrene (MW: 1,030,000)
2. PS, Polystyrene (MW: 152,000)
3. PS, Polystyrene (MW: 66,000)
4. PS, Polystyrene (MW: 22,000)
5. PS, Polystyrene (MW: 5,050)
6. PS, Polystyrene (MW: 580)
7. Ethylbenzene
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x n Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Each one, two and three units of GPC LF-404, were used for the analysis of epoxy resin. Only a single unit of LF-404 can determine a broad range of molecular weights from 300 to 2,000,000. However the resolution is higher depending on the number of columns, so it is better to use several units of columns for obtaining more accurate molecular weight distribution.
Sample : 0.2% EPON1009, 10micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x n Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Styrene butyl methacrylate copolymer was analyzed using GPC LF-404 (a column for organic SEC (GPC)).
Please refer to OUTLINE of LF-804.
Sample : 0.2% Styrene butyl methacrylate copolymer, 10micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2 Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Poly(methyl methacrylate) was analyzed using GPC LF-404 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Poly(methyl methacrylate) (PMMA), 10micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2 Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Ethylene vinyl acetate copolymer (28% vinyl acetate) was analyzed using GPC LF-404 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Ethylene vinyl acetate copolymer, 10micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2 Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
1,2-Polybutadiene was analyzed using GPC LF-404 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% 1,2-Polybutadiene, 10micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2 Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Phenoxy resin was analyzed using GPC LF-604 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Phenoxy resin, 20micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : DMF Flow rate : 0.5mL/min Detector : Shodex RI(small cell volume) Column temp. : 50deg-C
Poly(vinyl butyral) was analyzed using GPC LF-604 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Poly(vinyl butyral), 20micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : DMF Flow rate : 0.5mL/min Detector : Shodex RI(small cell volume) Column temp. : 50deg-C
Nylon6/6 was analyzed using GPC LF-604 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample: 20micro-L
0.2% Polyamide, Polyhexamethylene adipamide, Nylon6/6
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : 5mM CF3COONa in HFIP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Nylon6 was analyzed using GPC LF-604 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Polyamide, Polycaprolactam, Nylon6, 20micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : 5mM CF3COONa in HFIP Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Polylactic acid which is one of the biodegradable polymer was analyzed using GPC LF-604 (a column for organic SEC (GPC)). Please refer to OUTLINE of LF-804.
Sample : 0.2% Polylactic acid, (Biodegradable polymer) , 50micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2 Eluent : Chloroform Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
The calibration curves for downsized organic SEC (GPC) columns using PMMA are shown. In the case of the analysis for polymer whose MW is 1,000,000 or less, GPC LF-604 or LF-404 is suitable. When it is needed to separate low MW polymer and high MW polymer and low MW polymer is the purpose for the analysis, GPC HFIP-603 is suitable.
Please refer to Comparison of Analysis of PMMA with Down-sized SEC Columns
Sample : PMMA, Poly(methyl methacrylate) standards, 50micro-L
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2
Shodex GPC LF-404 (4.6mmID*250mm) x 2
Shodex GPC KF-405LHQ (4.6mmID*250mm) x 2
Shodex GPC KF-406LHQ (4.6mmID*250mm) x 2
Shodex GPC HFIP-603 (6.0mmID*150mm) x 2
Shodex GPC HFIP-606M (6.0mmID*150mm) x 2
Eluent : 5mM CF3COONa in HFIP
Flow rate : 0.3mL/min
Detector : Shodex RI(small cell volume)
Column temp. : 40deg-C
The calibration curves for downsized organic SEC (GPC) columns using PMMA are shown. In the case of the analysis for polymer whose MW is 1,000,000 or less, GPC LF-604 or LF-404 is suitable. When it is needed to separate low MW polymer and high MW polymer and low MW polymer is the purpose for the analysis, GPC HFIP-603 is suitable.
Please refer to Comparison of Calibration Curves for Down-sized SEC Columns (HFIP Eluent: PMMA)
Sample : 50micro-L
1. PMMA, Poly(methyl methacrylate) (MW: 1,944,000)
2. PMMA, Poly(methyl methacrylate) (MW: 281,700)
3. PMMA, Poly(methyl methacrylate) (MW: 79,250)
4. PMMA, Poly(methyl methacrylate) (MW: 13,300)
5. PMMA, Poly(methyl methacrylate) (MW: 1,960)
Columns : Shodex GPC LF-604 (6.0mmID*150mm) x 2
Shodex GPC LF-404 (4.6mmID*250mm) x 2
Shodex GPC KF-405LHQ (4.6mmID*250mm) x 2
Shodex GPC KF-406LHQ (4.6mmID*250mm) x 2
Shodex GPC HFIP-603 (6.0mmID*150mm) x 2
Shodex GPC HFIP-606M (6.0mmID*150mm) x 2
Eluent : 5mM CF3COONa in HFIP
Flow rate : 0.3mL/min
Detector : Shodex RI(small cell volume)
Column temp. : 40deg-C
Polyacetal was analyzed using GPC LF-404 (a column for organic SEC (GPC)).
Sample : Polyacetal, 20micro-L
Column : Shodex GPC LF-404 (4.6mmID*250mm) Eluent : 5mM CF3COONa in HFIP Flow rate : 0.15mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Poly(ethylene terephthalate) was analyzed using GPC LF-404 (a column for organic SEC (GPC)).
Sample : PET, Poly(ethylene terephthalate), 20micro-L
Column : Shodex GPC LF-404 (4.6mmID*250mm) Eluent : 5mM CF3COONa in HFIP Flow rate : 0.15mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C
Polyurethane was analyzed by organic solvent SEC (GPC) column, GPC LF-404.
Sample : Polyurethane(Fluka) 0.1%, 20micro-L
Columns : Shodex GPC LF-404 (4.6mmID*250mm) x 2 Eluent : THF Flow rate : 0.3mL/min Detector : Shodex RI(small cell volume) Column temp. : 40deg-C