Aflatoxins were analyzed with Silica C18M 4E column after the sample pretreatment.

Sample : 5micro-g/L each, 20micro-L

(TFA method) (PR method)
1. Derived Aflatoxin G1 1. Aflatoxin G2
2. Derived Aflatoxin B1 2. Aflatoxin G1
3. Aflatoxin G2 3. Aflatoxin B2
4. Aflatoxin B2 4. Aflatoxin B1
(TFA method) (PR method)
Column : Shodex Silica C18M 4E (4.6mmID*250mm) Column : Shodex Silica C18M 4E (4.6mmID*250mm)
Eluent : CH3CN/CH3OH/H2O=10/30/60 Eluent : CH3OH/H2O=40/60
Flow rate : 1.0mL/min Flow rate : 0.7mL/min
Detector : Fluorescence(Ex.365nm, Em.450nm) Detector : Fluorescence(Ex.365nm, Em.450nm)
Column temp. : 40deg-C Column temp. : 40deg-C

Deoxynivalenol(DON) and Nivalenol(NIV) in grains were analyzed with Silica C18M 4E after the sample pretreatment.

Sample : 20micro-L
(A) Standard solution (The amount of DON and NIV are 10ng each.)
(B) Sample solution of Wheat spiked with DON and NIV at 1micro-g/mL each

Column      : Shodex Silica C18M 4E (4.6mmID*250mm)
Eluent      : H2O/CH3OH/CH3CN=90/5/5
Flow rate   : 1.0mL/min
Detector    : UV(220nm)
Column temp.: 40deg-C
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