Organic SEC (GPC) Columns

Selection of Organic SEC (GPC) Columns

Shodex organic SEC (GPC) columns have a history of more than twenty years and have been used numerous customers all over the world. The current series is of an improved type based on the long term experience and the latest technology.
Five types of GPC columns packed with different solvents are available to avoid solvent replacement. Moreover, custom columns packed with other solvents such as quinoline, o-dichlorobenzene, ethyl acetate, tetrachloroethane and dimethylacetamide are available uponyour request.

The following columns are availble as the standard column.

1) In-column solvents

THF	KF-800, KF-600, KF-400HQ, LF series
Chloroform	K-800 series
DMF	KD-800 series
HFIP	HFIP-800, HFIP-600 series
Toluene	UT-800, HT-800, AT-806MS series

2) Types

Mixed gel columns	Mixed gel type columns are packed with mixture of gels which are different in pore size are to have wide linear range in molecular weight calibration curves from oligomers to macromolecules. The mixed gel columns have “M” at the end of the column name.
Linear type columns (1)	Linear type columns are specially designed to have wider linear range in molecular weight calibration curves than that of mixed gel columns. Linear type columns are highly recommended for correcting non-linear sections of molecular weight calibration curves of ordinary columns. It is highly recommended that linear type columns than ordinary type columns or mixed gel columns be used when both of them are available. The linear type columns have “L” at the end of the column name.
Linear type columns (2)	Linear type columns with special designed packing material.
Semi-micro GPC Columns	Small volume type columns are of 4.6mm ID column. The internal volume of 4.6mm ID column is about one third of 8.0mm ID column. Smaller particle size enables higher-resolution fractionation. Detection sensitivity is about four times higher than that of standard GPC columns. A single column can substitute for conventional analysis using two or more coupled columns, which shortens analysis time.
Downsized GPC Columns	Small volume type columns are of 6.0mm ID column. The internal volume of 6.0mm ID column is about one third of 8.0mm ID column. With small volume type column, by reducing the flow rate to one third, the same separation as with 8.0m ID columns can be obtained. And, the consumption of eluent will be also one third. Small volume type columns are good for reducing the consumption of potentially-environmentally-damaging organic solvents.
High Temperature Columns	The high temperature columns can be used for the GPC analysis at elevated temperature up to 230 Â°C.
Solvent-Peak Separation Column	Solvent-peak separation columns delay the elution of low-molecular components and can be used to separate a solvent peak or other troublesome peak to realize accurate measurement of molecular weight distribution. The use of the columns with 805L, 806L, 806M and 807L columns is recommended.

Column Specifications

Organic SEC (GPC) Columns: General Analysis

KF-800
K-800
KD-800

· Standard organic solvent SEC (GPC) columns
·Supports a wide range of applications from low to high-molecular-weight compounds

*Please refer to Replacement of In-Column Solvent
*Please refet to Solvent-peak Separation Columns for Organic SEC (GPC)
*The columns with ‘L’ or ‘M’ at the end of the column name are mixed-gel columns capable of a wide molecular weight distribution range of samples.

(Standard Columns)
KF-800 series shipping solvent : Tetrahydrofuran (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6028010 F6028020 F6028025 F6028030 F6027030 F6028040 F6027040 F6028050 F6027050 F6028060 F6028090 F6027060 F6028070 F6027070 F6700300	GPC KF-801 GPC KF-802 GPC KF-802.5 GPC KF-803 GPC KF-803L GPC KF-804 GPC KF-804L GPC KF-805 GPC KF-805L GPC KF-806 GPC KF-806M GPC KF-806L GPC KF-807 GPC KF-807L GPC KF-G	> 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 11,000 > 11,000 > 11,000 > 13,000 > 11,000 > 6,000 > 6,000 Guard column	1,500 5,000 20,000 70,000 70,000 400,000 400,000 4,000,000 4,000,000 (20,000,000) (20,000,000) (20,000,000) (200,000,000) *(200,000,000) –	6 6 6 6 6 7 7 10 10 10 10 10 18 18 8	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 4.6 x 10
F6709350	GPC KF-800D	Solvent-peak separation column	–	10	8.0 x 100

*( ): Estimated value
For all products
Recommended flow rate: 0.5 to 1.0mL/min
Max. usable flow rate: 2.0mL/min
Max. usable pressure: 3.5 MPa
Max. usable temperature: 60deg-C

K-800 series shipping solvent : Chloroform

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6028110 F6028120 F6028125 F6028130 F6028194 F6028140 F6028195 F6028150 F6028196 F6028160 F6028190 F6028197 F6028170 F6028198 F6700401	GPC K-801 GPC K-802 GPC K-802.5 GPC K-803 GPC K-803L GPC K-804 GPC K-804L GPC K-805 GPC K-805L GPC K-806 GPC K-806M GPC K-806L GPC K-807 GPC K-807L GPC K-G	> 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 18,000 > 11,000 > 11,000 > 11,000 > 13,000 > 11,000 > 6,000 > 6,000 Guard column	1,500 5,000 20,000 70,000 70,000 400,000 400,000 4,000,000 4,000,000 (20,000,000) (20,000,000) (20,000,000) (200,000,000) *(200,000,000) –	6 6 6 6 6 7 7 10 10 10 10 10 18 18 8	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 4.6 x 10
F6709450	GPC K-800D	Solvent-peak separation column	–	10	8.0 x 100

*( ): Estimated value
For all products
Recommended flow rate: 0.5 to 1.0mL/min
Max. usable flow rate: 2.0mL/min
Max. usable pressure: 3.5 MPa
Max. usable temperature: 60deg-C

KD-800 series shipping solvent : Dimethylformamide (DMF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (PEG/PEO)	Particle size (micron)	Size(mm) ID x Length
F6028210 F6028220 F6028225 F6028230 F6028240 F6028250 F6028260 F6028290 F6028270 F6700411	GPC KD-801 GPC KD-802 GPC KD-802.5 GPC KD-803 GPC KD-804 GPC KD-805 GPC KD-806 GPC KD-806M GPC KD-807 GPC KD-G	> 17,000 > 17,000 > 17,000 > 17,000 > 17,000 > 11,000 > 11,000 > 13,000 > 6,000 Guard column	2,500 5,000 20,000 70,000 400,000 4,000,000 (20,000,000) (20,000,000) *(200,000,000) –	6 6 6 6 7 10 10 10 18 8	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 4.6 x 10

*( ): Estimated value
For all products
Recommended flow rate: 0.5 to 1.0mL/min
Max. usable flow rate: 1.5mL/min
Max. usable pressure: 3.5 MPa
Max. usable temperature: 60deg-C

Organic SEC (GPC) Columns : High Performance Analysis

KF-400HQ

· About 1.5-times better separation performance than standard columns, with higher resolution
· About 4-times better sensitivity than that of standard columns, supporting high sensitivity analysis
· the amount od solvent used is reduced to about a third
· Improved applicability of solvent repracement

*Please refer to Replacement of In-Column Solvent
*The columns with ‘L’ at the end of the column name are mixed-gel columns capable of a wide molecular weight distribution range of samples.
*Use of the KF-400HQ series with semi-micro type devices is recommended.

(High performance semi-micro columns)
KF-400HQ series shipping solvent : Tetrahydrofulan (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6028111 F6028112 F6028114 F6028116 F6028118 F6028119 F6028122 F6700300	GPC KF-401HQ GPC KF-402HQ GPC KF-402.5HQ GPC KF-403HQ GPC KF-404HQ GPC KF-405LHQ GPC KF-406LHQ GPC KF-G	> 25,000 > 25,000 > 25,000 > 25,000 > 25,000 > 10,000 > 10,000 Guard column	1,500 5,000 20,000 70,000 400,000 4,000,000 *(20,000,000) –	3 3 3 3 3 10 10 8	4.6 x 250 4.6 x 250 4.6 x 250 4.6 x 250 4.6 x 250 4.6 x 250 4.6 x 250 4.6 x 10

*( ): Estimated value
For all products
Max. usable pressure: (KF-401HQ to KF-404HQ) 7MPa, (KF-405LHQ, KF-406L) 0.5MPa
Recommended flow rate: 0.2 to 0.3mL/min
Max. usable flow rate: 0.5mL/min
Max. usable temperature: 45deg-C

Organic SEC (GPC) Columns: Rapid Analysis

KF-600

· Approxymately half of the analysis time required standard columns
· The amount of solvent used is reduced to abaout a third
· Improved applicability of solvent replacement

Please refer to Replacement of In-Column Solvent
*The columns with ‘M’ at the end of the column name are mixed-gel columns capable of a wide molecular weight distribution range of samples.
*Use of the KF-600 series with semi-micro type devices is recommended.

(Rapid analysis downsized columns)
KF-600 series shipping solvent : Tetrahydrofulan (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6028091 F6028092 F6028093 F6028094 F6028095 F6028096 F6028097 F6028098 F6028099 F6700300	GPC KF-601 GPC KF-602 GPC KF-602.5 GPC KF-603 GPC KF-604 GPC KF-605 GPC KF-606 GPC KF-606M GPC KF-607 GPC KF-G	> 17,000 > 17,000 > 17,000 > 17,000 > 16,000 > 7,000 > 7,000 > 8,000 > 5,000 Guard column	1,500 5,000 20,000 70,000 400,000 4,000,000 (20,000,000) (20,000,000) *(200,000,000) –	3 3 3 3 3 10 10 10 18 8	6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 4.6 x 10

*( ): Estimated value
For all products
Max. usable pressure: (KF-601 to KF-604) 6.0MPa, (KF-605 to KF-607) 0.6MPa
Recommended flow rate: 0.4 to 0.6mL/min
Max. usable flow rate: 0.8mL/min
Max. usable temperature: 45deg-C
Figures in parentheses are estimated values.

Organic SEC (GPC) Columns: Linear Calibration Type

· Employs a special packing materials with a wide pore size distribution (multi pore type)
· Enables analysis over a broad range of molecular weights (100 to2 million)
· Highly linear calibration curve without infection points
· Molecular weight distribution can be determined with high precision
· Column for rapid analysis (LF-604) and columns for high performance analysis (LF-404) enabling reduction in solvent use are also available

Please refer to Replacement of In-Column Solvent
*Use of the LF-604 and the LF-404 with semi-micro type devices is recommended.

(Standard column) shipping solvent : Tetrahydrofulan (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6021041 F6709621	GPC LF-804 GPC LF-G	> 17,000 Guard column	2,000,000 –	6 6	8.0 x 300 4.6 x 10

Product name	Max. usable pressure(MPa)	Recommended flow rate(mL/min)	Max. usable flow rate(mL/min)	Max. usable temp. (deg-C)
LF-804 LF-G	3.5 –	1.0 –	2.0 –	20 to 60 20 to 60

(Rapid analysis downsized column) shipping solvent : Tetrahydrofulan (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6021042 F6709621	GPC LF-604 GPC LF-G	> 9,000 Guard column	2,000,000 –	6 6	6.0 x 150 4.6 x 10

Product name	Max. usable pressure(MPa)	Recommended flow rate(mL/min)	Max. usable flow rate(mL/min)	Max. usable temp. (deg-C)
LF-604 LF-G	3.5 –	0.5 –	1.0 –	20 to 60 20 to 60

(High-perfomance semi-micro column) shipping solvent : Tetrahydrofulan (THF)

Product code	Product name	Plate number (TPN/column)	Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6021043 F6709621	GPC LF-404 GPC LF-G	> 14,000 Guard column	2,000,000 –	6 6	4.0 x 250 4.6 x 10

Product name	Max. usable pressure(MPa)	Recommended flow rate(mL/min)	Max. usable flow rate(mL/min)	Max. usable temp. (deg-C)
LF-404 LF-G	3.5 –	0.3 –	0.6 –	20 to 60 20 to 60

Organic SEC (GPC) Columns: HFIP SolventType

HFIP-800	· Columns exclusively for use with hexafluoroisopropanol (HFIP)
HFIP-600	· Rapid analysis, solvent-saving type

*The columns with ‘M’ at the end of the column name are mixed-gel columns capable of a wide molecular weight distribution range of samples.

(Standard Columns)
HFIP-800 series shipping solvent : Hexafluoroisopropanol (HFIP)

Product code	Product name	Plate number (TPN/column)	Exclusion range (PMMA)	Particle size (micron)	Size(mm) ID x Length
F6028530 F6028540 F6028550 F6028560 F6028590 F6028570 F6700500	GPC HFIP-803 GPC HFIP-804 GPC HFIP-805 GPC HFIP-806 GPC HFIP-806M GPC HFIP-807 GPC HFIP-LG	> 12,000 > 12,000 > 10,000 > 10,000 > 10,000 > 4,000 Guard column	30,000 100,000 1,000,000 (10,000,000) (10,000,000) *(100,000,000) –	10 7 10 10 10 18 15	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 50

*( ): Estimated value
For all products
Max. usable pressure: 4.0 MPa
Recommended flow rate: 0.5 to 1.0mL/min
Max. usable flow rate: 1.5mL/min
Max. usable temperature: 50deg-C

(Rapid analysis downsized columns)
HFIP-600 series shipping solvent : Hexafluoroisopropanol (HFIP)

Product code	Product name	Plate number (TPN/column)	Exclusion range (PMMA)	Particle size (micron)	Size(mm) ID x Length
F6021030 F6021040 F6021050 F6021060 F6021080 F6021070 F6700511	GPC HFIP-603 GPC HFIP-604 GPC HFIP-605 GPC HFIP-606 GPC HFIP-606M GPC HFIP-607 GPC HFIP-G	> 12,000 > 12,000 > 5,000 > 5,000 > 6,000 > 3,000 Guard column	30,000 100,000 1,000,000 (10,000,000) (10,000,000) *(100,000,000) –	3 3 10 10 10 18 8	6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 6.0 x 150 4.6 x 10

*( ): Estimated value
For all products
Max. usable pressure: 6.0MPa (HFIP-603, 604), 1.0MPa (HFIP-605 to 607)
Max. usable flow rate: 0.3mL/min (HFIP-603 to 604), 0.6mL/min (HFIP-605 to 607)
Max. usable temperature: 50deg-C

Organic SEC (GPC) Columns: High Temperature / Ultra High Temperature Analysis

HT-800	· Varied product line up to support a wide range of molecukar weights
UT-800	· Dedicated to SEC analysis at high/ultra high temperatures with a maximum usable temperature of 210 deg-C · Suitable for the analysis of supermolecule-containing samples

*The columns with ‘M’ at the end of the column name are mixed-gel columns capable of a wide molecular weight distribution range of samples.

(Standard Columns)

Product code	Product name	Plate number (TPN/column)	*Exclusion limit (Polystyrene)	Particle size (micron)	Size(mm) ID x Length
F6208700 F6208710 F6208720 F6208730 F6208740 F6208770 F6709410	GPC HT-803 GPC HT-804 GPC HT-805 GPC HT-806 GPC HT-806M GPC HT-807 GPC HT-G	> 7,000 > 7,000 > 7,000 > 7,000 > 7,000 > 4,000 Guard column	70,000 400,000 4,000,000 (20,000,000) (20,000,000) **(200,000,000) –	13 13 13 13 13 18 13	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 50
F6208600 F6208610 F6208620 F6709400	GPC UT-802.5 GPC UT-806M GPC UT-807 GPC UT-G	> 4,400 > 4,400 > 3,300 Guard column	20,000 (20,000,000) (200,000,000) –	30 30 30 30	8.0 x 300 8.0 x 300 8.0 x 300 8.0 x 50
F6208390 F6700280	GPC AT-806MS GPC AT-G	> 6,000 Guard column	**(20,000,000) –	12 15	8.0 x 250 8.0 x 50

Product name	Max. usable pressure(MPa)	Recommended flow rate(mL/min)	Max. usable flow rate(mL/min)	Usable temp. range (deg-C)	Shipping solvent
HT-803 HT-804 HT-805 HT-806 HT-806M HT-807 HT-G	1.5 1.5 1.5 1.5 1.5 1.5 –	0.5 to 1.0 0.5 to 1.0 0.5 to 1.0 0.5 to 1.0 0.5 to 1.0 0.5 to 1.0 –	1.5 1.5 1.5 1.5 1.5 1.5 –	100 to 150 100 to 150 100 to 150 100 to 150 100 to 150 100 to 150 –	Toluene Toluene Toluene Toluene Toluene Toluene Toluene
UT-802.5 UT-806M UT-807 UT-G	1.0 1.0 1.0 –	0.3 to 1.0 0.3 to 1.0 0.3 to 1.0 –	1.5 1.5 1.5 –	100 to 210 100 to 210 100 to 210 –	Toluene Toluene Toluene Toluene
AT-806MS AT-G	2.0 –	0.5 to 1.0 –	1.5 –	RT to 150 –	Toluene Toluene

*: Exclusionlimit was measured with THF
** ( ): Estimated value

Solvent-peak Separation Columns for Organic SEC (GPC)

KF-800D
K-800D

· Shifting the elution of monomers, polymer additives and solvent peak in low-molecular-weight region
· Reduces interferences to calculate the molecular weight distribution of polymers or oligomers
·Use this type of column in combination with a linear column

Use this type of column in combination with a linear column.

Product code	Product name	Column combination	Particle size (micron)	Size(mm) ID x Length	Shipping solvent
F6709350	GPC KF-800D	KF-805L ,KF-806L, KF-806M, KF-807L	10	8.0 x 100	THF
F6709450	GPC K-800D	K-805L, K-806L, K-806M, K-807L	10	8.0 x 100	Chloroform

Preparative Organic SEC (GPC) Columns

(Preparative columns)(Remark)

Product code	Product name	Plate number (TPN/column)	Particle size (micron)	Size(mm) ID x Length	Standard column
F6102301 F6102312 F6102315 F6102303 F6102304 F6102305 F6102306 F6102309 F6700407	GPC K-2001 GPC K-2002 GPC K-2002.5 GPC K-2003 GPC K-2004 GPC K-2005 GPC K-2006 GPC K-2006M GPC K-LG	> 18,000 > 18,000 > 18,000 > 18,000 > 14,000 > 10,000 > 10,000 > 10,000 Guard column	6 6 6 6 7 10 10 10 15	20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 8.0 x 50	K-801 K-802 K-802.5 K-803 K-804 K-805 K-806 K-806M Guard column
F6102401 F6102402 F6102425 F6102403 F6102404 F6102405 F6102406 F6102409 F6700406	GPC KF-2001 GPC KF-2002 GPC KF-2002.5 GPC KF-2003 GPC KF-2004 GPC KF-2005 GPC KF-2006 GPC KF-2006M GPC KF-LG	> 18,000 > 18,000 > 18,000 > 18,000 > 14,000 > 10,000 > 10,000 > 10,000 Guard column	6 6 6 6 7 10 10 10 15	20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 20.0 x 300 8.0 x 50	KF-801 KF-802 KF-802.5 KF-803 KF-804 KF-805 KF-806 KF-806M Guard column

Exclusion limits are as same as those of corresponding standard columns.
For all producs
Max. usable flow rate: 4.5mL/min
Max. usable pressure: 2.0MPa
Max. usable temperature: 60deg-C

(Preparative columns (Special order))(Remark)

Product code	Product name	Plate number (TPN/column)	Particle size (micron)	Size(mm) ID x Length	Standard column
F6102001 F6102002 F6102025 F6102003 F6102004 F6102005 F6102006 F6102009 F6700310	GPC H-2001 GPC H-2002 GPC H-2002.5 GPC H-2003 GPC H2004 GPC H-2005 GPC H-2006 GPC H2006M GPC H-G	> 13,000 > 13,000 > 13,000 > 13,000 > 13,000 > 13,000 > 13,000 > 12,000 Guard column	15 15 15 15 15 15 15 15 15	20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 20.0 x 500 8.0 x 50	K-801 K-802 K-802.5 K-803 K-804 K-805 K-806 K-806M Guard column

Product code	Product name	Particle size (micron)	Size(mm) ID x Length	Standard column
F6108010 F6108020 F6108025 F6108030 F6108040 F6700408	GPC KF-5001 GPC KF-5002 GPC KF-5002.5 GPC KF-5003 GPC KF-5004 GPC KF-LLG	15 15 15 15 15 15	50.0 x 300 50.0 x 300 50.0 x 300 50.0 x 300 50.0 x 300 20.0 x 100	KF-801 KF-802 KF-802.5 KF-803 KF-804 Guard column

Column Information

Sample Concentration for SEC Analysis

For SEC analysis, sample concentration is very important. The following is the pretreatment procedure of sample.

Basically, the sample should be dissolved in the same solvent that is to be used as the eluent.

1) Dissolving the sample in such solvent will make the blank peaks as small as possible.

2) In case the sample has a molecular weight of 1,000,000 minimum, soak it in such solvent for 12 to 24 hours to let it swell. After the swelling, stir the solvent gently for dissolution.
Strong agitation or use of an ultrasonic bath for dissolution will degrade such sample.

3) In case the sample is a polymer, its concentration in the solution and the injection volume should be 0.05 to 0.5% and 50 to 100micro-L, respectively.
If the concentration is higher, the retention volume of the sample will increase. The optimum concentration changes with the molecular weight and the viscosity. The following table gives the molecular weight vs. the optimum concentration.

Molecular weight	Sample concentration (W/V%)	Injection volume per column
Molecular weight	Sample concentration (W/V%)	KF-600 series	KF-800 series
< 5,000	less than 1.0	micro-L	to 100 micro-L
5,000 to 25,000	less than 0.5	micro-L
25,000 to 200,000	less than 0.25	30 micro-L
200,000 to 2,000,000	less than 0.1
> 2,000,000	less than 0.05

4) In case the sample is organic, it is desirable that the concentration be 1% maximum and the injection volume, 50micro-L maximum.

Features of High Temperature SEC Column

The GPC UT-800 columns have been developed to prevent shear degradation in the high molecular weight range. The existence of shear degradation can be confirmed using a multi angle laser light scattering(MALS) detector, which has high sensitivity in the high molecular weight range and can measure absolute molecular weight. This shows the comparison of results of analysis of high density polyethylene(HDPE) using conventional columns and UT-806M columns. Detection was performed using MALS and RI detectors. With conventional columns, the highest molecular weight portion detected was around 5,000,000. On the contrary, with UT-806M, the highest molecular weight portion was around 20,000,000 and the results show that high molecular weight portions which cannot be measured using conventional columns exist. Regarding weighted mean molecular weight values(Mw) obtained from GPC calibration curves, Mw using UT-806M columns is higher than tat using conventional columns. From the above results, it can be concluded that shear degradation in the high molecular weight range occurred with conventional columns, but, not with UT-806M columns.

Product name	Particle size (microm)	Mw of HDP
Product name	Particle size (microm)	A	B
GPC UT-806M	30	1,000,000	468,000
Conventional column	12	712,000	333,000
(A) Mw(PS) : obtained from calibration curve (B) Mw(PE) : Mw(PE)=A x 0.468

Sample : 0.3% HDPE-A, High density polyethylene, 500micro-L

Columns      : Shodex GPC UT-806M (8.0mmID*300mm) x 2
Eluent       : 0.1% BHT*/ODCB
Flow rate    : 1.0mL/min
Detector     : RI, MALS(Multi angle laser light scattering)
Column temp. : 145deg-C

*BHT: Butylhydroxytoluene

Replacement of In-Column Solvent

Please refer to the following tables for the classification of solvents in terms of capability of being replaced with original in-column solvent. However, frequent replacement of in-column solvent may shorten column life, and is, therefore, not recommended. We can replace the in-column solvent with another solvent such as o-dichlorobenzene, ethyl acetate, dimethylacetamide or methylpyrrolidon upon your request.

(Standard Organic SEC (GPC) columns)

(Downsized GPC columns and Semi-micro GPC columns)

(Linear Type : LF series)

Request information on Shodex Columns for your Laboratory